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Sticholysins, pore-forming proteins from a marine anemone can induce maturation of dendritic cells through a TLR4 dependent-pathway.
Laborde, Rady J; Ishimura, Mayari E; Abreu-Butin, Lianne; Nogueira, Catarina V; Grubaugh, Daniel; Cruz-Leal, Yoelys; Luzardo, María C; Fernández, Audry; Mesa, Circe; Pazos, Fabiola; Álvarez, Carlos; Alonso, María E; Starnbach, Michael N; Higgins, Darren E; Fernández, Luis E; Longo-Maugéri, Ieda M; Lanio, María E.
Afiliación
  • Laborde RJ; Laboratory of Toxins and Liposomes, Center for Protein Studies, Faculty of Biology, University of Havana (UH), Lab UH-CIM, Havana, 10400, Cuba. Electronic address: radylq@fbio.uh.cu.
  • Ishimura ME; Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), 04023-062, São Paulo, Brazil. Electronic address: mayariei@hotmail.com.
  • Abreu-Butin L; Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), 04023-062, São Paulo, Brazil.
  • Nogueira CV; Department of Microbiology and Immunobiology of Harvard Medical School, Harvard University, MA, USA. Electronic address: catsnogueira@gmail.com.
  • Grubaugh D; Department of Microbiology and Immunobiology of Harvard Medical School, Harvard University, MA, USA. Electronic address: grubaugh@vet.upenn.edu.
  • Cruz-Leal Y; Laboratory of Toxins and Liposomes, Center for Protein Studies, Faculty of Biology, University of Havana (UH), Lab UH-CIM, Havana, 10400, Cuba. Electronic address: yoelyscruz@gmail.com.
  • Luzardo MC; Laboratory of Toxins and Liposomes, Center for Protein Studies, Faculty of Biology, University of Havana (UH), Lab UH-CIM, Havana, 10400, Cuba. Electronic address: mcluzardo@fbio.uh.cu.
  • Fernández A; Immunobiology Division, Center of Molecular Immunology (CIM), Havana, 11600, Cuba. Electronic address: audryfg81@gmail.com.
  • Mesa C; Immunobiology Division, Center of Molecular Immunology (CIM), Havana, 11600, Cuba. Electronic address: circe@cimab.cu.
  • Pazos F; Laboratory of Toxins and Liposomes, Center for Protein Studies, Faculty of Biology, University of Havana (UH), Lab UH-CIM, Havana, 10400, Cuba. Electronic address: fpazos@fbio.uh.cu.
  • Álvarez C; Laboratory of Toxins and Liposomes, Center for Protein Studies, Faculty of Biology, University of Havana (UH), Lab UH-CIM, Havana, 10400, Cuba. Electronic address: calvarez@fbio.uh.cu.
  • Alonso ME; Laboratory of Toxins and Liposomes, Center for Protein Studies, Faculty of Biology, University of Havana (UH), Lab UH-CIM, Havana, 10400, Cuba.
  • Starnbach MN; Department of Microbiology and Immunobiology of Harvard Medical School, Harvard University, MA, USA. Electronic address: starnbach@hms.harvard.edu.
  • Higgins DE; Department of Microbiology and Immunobiology of Harvard Medical School, Harvard University, MA, USA. Electronic address: darren_higgins@hms.harvard.edu.
  • Fernández LE; Immunobiology Division, Center of Molecular Immunology (CIM), Havana, 11600, Cuba. Electronic address: luis@cim.sld.cu.
  • Longo-Maugéri IM; Discipline of Immunology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), 04023-062, São Paulo, Brazil. Electronic address: imaugeri@unifesp.br.
  • Lanio ME; Laboratory of Toxins and Liposomes, Center for Protein Studies, Faculty of Biology, University of Havana (UH), Lab UH-CIM, Havana, 10400, Cuba. Electronic address: mlanio@fbio.uh.cu.
Mol Immunol ; 131: 144-154, 2021 03.
Article en En | MEDLINE | ID: mdl-33422341
Sticholysins (Sts) I and II (StI and StII) are pore-forming proteins (PFPs), purified from the Caribbean Sea anemone Stichodactyla helianthus. StII encapsulated into liposomes induces a robust antigen-specific cytotoxic CD8+ T lymphocytes (CTL) response and in its free form the maturation of bone marrow-derived dendritic cells (BM-DCs). It is probable that the latter is partially supporting in part the immunomodulatory effect on the CTL response induced by StII-containing liposomes. In the present work, we demonstrate that the StII's ability of inducing maturation of BM-DCs is also shared by StI, an isoform of StII. Using heat-denatured Sts we observed a significant reduction in the up-regulation of maturation markers indicating that both PFP's ability to promote maturation of BM-DCs is dependent on their conformational characteristics. StII-mediated DC maturation was abrogated in BM-DCs from toll-like receptor (TLR) 4 and myeloid differentiation primary response gene 88 (MyD88)-knockout mice but not in cells from TLR2-knockout mice. Furthermore, the antigen-specific CTL response induced by StII-containing liposomes was reduced in TLR4-knockout mice. These results indicate that StII, and probably by extension StI, has the ability to induce maturation of DCs through a TLR4/MyD88-dependent pathway, and that this activation contributes to the CTL response generated by StII-containing liposomes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Dendríticas / Venenos de Cnidarios / Receptor Toll-Like 4 Límite: Animals Idioma: En Revista: Mol Immunol Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Dendríticas / Venenos de Cnidarios / Receptor Toll-Like 4 Límite: Animals Idioma: En Revista: Mol Immunol Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido