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Acute and chronic infection of H. pylori caused the difference in apoptosis of gastric epithelial cells.
Liu, Jia-Fei; Guo, Dong; Kang, En-Ming; Wang, Yu-Shan; Gao, Xiao-Zhong; Cong, Hai-Yan; Liu, Peng; Zhang, Nai-Qian; Wang, Ming-Yi.
Afiliación
  • Liu JF; Department of Central Lab, Weihai Municipal Hospital, Shandong University. Weihai, Shandong, 264200, PR China; Department of Medical Laboratory Science, Weifang Medical University, Weifang, Shandong, 261000, PR China.
  • Guo D; Department of Central Lab, Weihai Municipal Hospital, Shandong University. Weihai, Shandong, 264200, PR China.
  • Kang EM; Department of Central Lab, Weihai Municipal Hospital, Shandong University. Weihai, Shandong, 264200, PR China.
  • Wang YS; School of Laboratory Medicine, Dalian Medical University, Dalian, Liaoning, 116044, PR China.
  • Gao XZ; Department of Gastroenterology, Weihai Municipal Hospital, Shandong University. Weihai, Shandong, 264200, PR China.
  • Cong HY; Department of Central Lab, Weihai Municipal Hospital, Shandong University. Weihai, Shandong, 264200, PR China.
  • Liu P; Department of Central Lab, Weihai Municipal Hospital, Shandong University. Weihai, Shandong, 264200, PR China.
  • Zhang NQ; School of Mathematics and Statistics, Shandong University, Weihai, Shandong, 264209, PR China. Electronic address: nqzhang@email.sdu.edu.cn.
  • Wang MY; Department of Central Lab, Weihai Municipal Hospital, Shandong University. Weihai, Shandong, 264200, PR China; Department of Medical Laboratory Science, Weifang Medical University, Weifang, Shandong, 261000, PR China. Electronic address: wangmingyi1973@outlook.com.
Microb Pathog ; 150: 104717, 2021 Jan.
Article en En | MEDLINE | ID: mdl-33421608
Helicobacter pylori (H. pylori) is one of the most important pathogenic bacteria associated with various gastrointestinal diseases. At present, its apoptotic or antiapoptotic mechanism on gastric epithelial cells remains unknown and needs further illustrated. In this study, acute infection model (H. pylori and GES-1 cells were co-cultured for 24 h at a multiplicity of infection MOI of 100:1) and chronic infection model (GES-1 cells were infected repeatedly every 24 h at a multiplicity of infection MOI of 100:1 for approximately 8 weeks) were established, respectively. the chronic H. pylori infected GES-1 cells underwent a typically morphological change and Western Blot results showed that there was slight decrease in expression of E-cadherin, and obvious increase in expression of Vimentin. Apoptosis of these two models were analyzed by flow cytometry compared with the control cells, meanwhile, apoptosis associated markers (Bcl-xL, Bcl-2, Bax, etc) were detected by Western blot, additional in clinical H. pylori-positive gastric cancer tissues. Results showed that compared with the control cells, acute infection of H. pylori significantly accelerated the apoptosis of GES-1, increased the expression of Bax and Cleaved caspase-3, down-regulated expression of Bcl-xL and Bcl-2. Moreover, an opposite result was found in chronic infection of model and clinical gastric cancer tissues, and enhanced expression of NF-κB p65. Taken together, these findings suggest that H. pylori infection plays differential effects on apoptosis of gastric epithelial cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido