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Enzymatic degradation of ginkgolic acids by laccase immobilized on core/shell Fe3O4/nylon composite nanoparticles using novel coaxial electrospraying process.
Chen, Hung-Yueh; Ting, Yuwen; Kuo, Hsing-Chun; Hsieh, Chang-Wei; Hsu, Hsien-Yi; Wu, Chun-Nan; Cheng, Kuan-Chen.
Afiliación
  • Chen HY; Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
  • Ting Y; Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
  • Kuo HC; Chang Gung Memorial Hospital, Chiayi 613, Taiwan; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33305, Taiwan; Department of Nursing, Division of Basic Medical Sciences, Chang Gung University of Science and Technology, Chiayi 61363, Taiwan; C
  • Hsieh CW; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 145, Taiwan.
  • Hsu HY; School of Energy and Environment & Department of Materials Science and Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong, China; Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China.
  • Wu CN; Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan. Electronic address: wulalaworld@gmail.com.
  • Cheng KC; Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan; Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404332, Taiwan; Dep
Int J Biol Macromol ; 172: 270-280, 2021 Mar 01.
Article en En | MEDLINE | ID: mdl-33418049
Enzyme immobilization can increase enzyme reusability to reduce cost of industrial production. Ginkgo biloba leaf extract is commonly used for medical purposes, but it contains ginkgolic acid, which has negative effects on human health. Here, we report a novel approach to solve the problem by degrading the ginkgolic acid with immobilized-laccase, where core/shell composite nanoparticles prepared by coaxial electrospraying might be first applied to enzyme immobilization. The core/shell Fe3O4/nylon 6,6 composite nanoparticles (FNCNs) were prepared using one-step coaxial electrospraying and can be simply recovered by magnetic force. The glutaraldehyde-treated FNCNs (FNGCNs) were used to immobilize laccase. As a result, thermal stability of the free laccase was significantly improved in the range of 60-90 °C after immobilization. The laccase-immobilized FNGCNs (L-FNGCNs) were applied to degrade the ginkgolic acids, and the rate constants (k) and times (τ50) were ~0.02 min-1 and lower than 39 min, respectively, showing good catalytic performance. Furthermore, the L-FNGCNs exhibited a relative activity higher than 0.5 after being stored for 21 days or reused for 5 cycles, showing good storage stability and reusability. Therefore, the FNGCNs carrier was a promising enzyme immobilization system and its further development and applications were of interest.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Fúngicas / Salicilatos / Ginkgo biloba / Lacasa / Óxido Ferrosoférrico / Nanopartículas de Magnetita Idioma: En Revista: Int J Biol Macromol Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Países Bajos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Fúngicas / Salicilatos / Ginkgo biloba / Lacasa / Óxido Ferrosoférrico / Nanopartículas de Magnetita Idioma: En Revista: Int J Biol Macromol Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Países Bajos