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Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics.
Cao, Simin; Li, Haoyang; Zhao, Zenan; Zhang, Sanjun; Chen, Jinquan; Xu, Jianhua; Knutson, Jay R; Brand, Ludwig.
Afiliación
  • Cao S; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, China.
  • Li H; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, China.
  • Zhao Z; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, China.
  • Zhang S; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, China.
  • Chen J; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, China.
  • Xu J; State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, China.
  • Knutson JR; Laboratory for Advanced Microscopy and Biophotonics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • Brand L; Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
Molecules ; 26(1)2021 Jan 03.
Article en En | MEDLINE | ID: mdl-33401638
In this review, the experimental set-up and functional characteristics of single-wavelength and broad-band femtosecond upconversion spectrophotofluorometers developed in our laboratory are described. We discuss applications of this technique to biophysical problems, such as ultrafast fluorescence quenching and solvation dynamics of tryptophan, peptides, proteins, reduced nicotinamide adenine dinucleotide (NADH), and nucleic acids. In the tryptophan dynamics field, especially for proteins, two types of solvation dynamics on different time scales have been well explored: ~1 ps for bulk water, and tens of picoseconds for "biological water", a term that combines effects of water and macromolecule dynamics. In addition, some proteins also show quasi-static self-quenching (QSSQ) phenomena. Interestingly, in our more recent work, we also find that similar mixtures of quenching and solvation dynamics occur for the metabolic cofactor NADH. In this review, we add a brief overview of the emerging development of fluorescent RNA aptamers and their potential application to live cell imaging, while noting how ultrafast measurement may speed their optimization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Ácidos Nucleicos / Proteínas / Fluorescencia Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Ácidos Nucleicos / Proteínas / Fluorescencia Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza