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Steroid Receptor Coactivator-3 Is Required for Inhibition of the Intestinal Muscularis Inflammatory Response of Postoperative Ileus in Mice.
Zou, Kang; Chen, Wenbo; Dai, Jun; Mo, Pingli; Yu, Chundong; Xu, Jianming; Wu, Sangang; Zhuo, Rengong; Su, Guoqiang.
Afiliación
  • Zou K; Department of Gastrointestinal Surgery, Ward 3 Areas of Cancer Center, Cancer Hospital, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, China.
  • Chen W; Department of Gastrointestinal Surgery, Ward 3 Areas of Cancer Center, Cancer Hospital, Teaching Hospital of Fujian Medical University, Xiamen, 361003, Fujian, China.
  • Dai J; Department of Critical Care Medicine, the First Affiliated Hospital of Gannan Medical College, Ganzhou, 341000, China.
  • Mo P; Department of Cardiology, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China.
  • Yu C; Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, 361005, China.
  • Xu J; Department of Gastrointestinal Surgery, Ward 3 Areas of Cancer Center, Cancer Hospital, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, China.
  • Wu S; Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, 361005, China.
  • Zhuo R; Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, 361005, China.
  • Su G; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
Inflammation ; 44(3): 1145-1159, 2021 Jun.
Article en En | MEDLINE | ID: mdl-33398542
Inflammation theory has suggested that the pathogenesis of postoperative ileus (POI) involves the steroid receptor coactivator-3 (SRC-3). Therefore, we investigated the role of SRC-3 in the muscles of the small intestine using a mouse POI model. Here, we reported that intestinal manipulation (IM) significantly reduced the extent of phenol red migration in the entire gastrointestinal tract, and the calculated geometric center (GC) value in wild-type (WT) mice at 24 h after surgery was higher than that in the knockout (KO) mice and in the sham-operated control group. The expression of SRC-3 was upregulated in the mouse intestinal muscularis at 24 h after surgical manipulation, and the mRNA and protein levels of inflammatory cytokines were upregulated compared with those in the control group. At 24 h after IM, the number of neutrophils in the experimental group was significantly higher than that in the control group; in the IM group, the number of neutrophils in the SRC-3-/- mice was markedly higher than that in the WT mice. At 24 h after IM, the myeloperoxidase (MPO) activity in the experimental group was significantly higher than that in the control group. In the IM group, the MPO activity of the SRC-3-/- mice was markedly higher than that of the WT mice. In summary, proinflammatory cytokines, the number of neutrophils, and the MPO activity were significantly increased in the muscularis of the jejunum and ileum of KO mice after IM compared with those of the WT mice, indicating that SRC-3 might play a protective role in POI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complicaciones Posoperatorias / Citocinas / Mediadores de Inflamación / Ileus / Coactivador 3 de Receptor Nuclear / Motilidad Gastrointestinal / Intestino Delgado / Músculo Liso Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Inflammation Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complicaciones Posoperatorias / Citocinas / Mediadores de Inflamación / Ileus / Coactivador 3 de Receptor Nuclear / Motilidad Gastrointestinal / Intestino Delgado / Músculo Liso Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Inflammation Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos