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A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes.
Hibi, Makoto; Fukuda, Dai; Kenchu, Chihiro; Nojiri, Masutoshi; Hara, Ryotaro; Takeuchi, Michiki; Aburaya, Shunsuke; Aoki, Wataru; Mizutani, Kimihiko; Yasohara, Yoshihiko; Ueda, Mitsuyoshi; Mikami, Bunzo; Takahashi, Satomi; Ogawa, Jun.
Afiliación
  • Hibi M; Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan. hibi@pu-toyama.ac.jp.
  • Fukuda D; Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan. hibi@pu-toyama.ac.jp.
  • Kenchu C; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Nojiri M; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Hara R; Biotechnology Research Laboratories, Kaneka Corporation, 1-8 Miyamae, Takasago, Hyogo, 676-8688, Japan.
  • Takeuchi M; Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Aburaya S; Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Aoki W; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Mizutani K; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Yasohara Y; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Ueda M; Biotechnology Research Laboratories, Kaneka Corporation, 1-8 Miyamae, Takasago, Hyogo, 676-8688, Japan.
  • Mikami B; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Takahashi S; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Ogawa J; Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Commun Biol ; 4(1): 16, 2021 01 04.
Article en En | MEDLINE | ID: mdl-33398074
The high-valent iron-oxo species formed in the non-heme diiron enzymes have high oxidative reactivity and catalyze difficult chemical reactions. Although the hydroxylation of inert methyl groups is an industrially promising reaction, utilizing non-heme diiron enzymes as such a biocatalyst has been difficult. Here we show a three-component monooxygenase system for the selective terminal hydroxylation of α-aminoisobutyric acid (Aib) into α-methyl-D-serine. It consists of the hydroxylase component, AibH1H2, and the electron transfer component. Aib hydroxylation is the initial step of Aib catabolism in Rhodococcus wratislaviensis C31-06, which has been fully elucidated through a proteome analysis. The crystal structure analysis revealed that AibH1H2 forms a heterotetramer of two amidohydrolase superfamily proteins, of which AibHm2 is a non-heme diiron protein and functions as a catalytic subunit. The Aib monooxygenase was demonstrated to be a promising biocatalyst that is suitable for bioprocesses in which the inert C-H bond in methyl groups need to be activated.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhodococcus / Aminobutiratos / Oxigenasas de Función Mixta Idioma: En Revista: Commun Biol Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhodococcus / Aminobutiratos / Oxigenasas de Función Mixta Idioma: En Revista: Commun Biol Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido