Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?

Manukyan, Levon; Marinaki, Maria-Eleni; Mihranyan, Albert

Manukyan, Levon; Marinaki, Maria-Eleni; Mihranyan, Albert.

Afiliación

Manukyan L; Nanotechnology and Functional Materials, Department of Materials Science and Engineering, Uppsala University, Box 35, 751 03 Uppsala, Sweden.
Marinaki ME; Nanotechnology and Functional Materials, Department of Materials Science and Engineering, Uppsala University, Box 35, 751 03 Uppsala, Sweden.
Mihranyan A; Nanotechnology and Functional Materials, Department of Materials Science and Engineering, Uppsala University, Box 35, 751 03 Uppsala, Sweden.

ACS Appl Bio Mater ; 3(12): 8344-8351, 2020 Dec 21.

Article en En | MEDLINE | ID: mdl-33381749

RESUMEN

Virus safety of fetal bovine serum (FBS) is a critical issue for cell culture and clinical applications of cell therapies. The size exclusion filtration of FBS-supplemented cell culture media through small-size virus retentive filter paper is presented to investigate its effect on cell culture. A substantial proportion of proteins (ca. 45%) was removed by nanofiltration, yet important transport proteins (albumin, fetuins, macroglobulins, transferrin) were unaffected. The cell viability of Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK-293) cells that were grown in media supplemented with nanofiltered FBS was surprisingly high, despite the observed protein losses. Protein depletion following nanofiltration resulted in detectable levels of autophagy markers.

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Appl Bio Mater Año: 2020 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos

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