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A highly sensitive and versatile transcription immunoassay using a DNA-encoding tandem repetitive light-up aptamer.
Sim, Jieun; Baek, Min-Seok; Lee, Kyung-Ho; Kim, Dong-Myung; Byun, Ju-Young; Shin, Yong-Beom.
Afiliación
  • Sim J; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, North Korea; Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, 34113, North Korea; BioNano Health Guard Research Center (H
  • Baek MS; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764, North Korea.
  • Lee KH; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764, North Korea.
  • Kim DM; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764, North Korea.
  • Byun JY; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, North Korea. Electronic address: bjy8349@kribb.re.kr.
  • Shin YB; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, North Korea; Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, 34113, North Korea; BioNano Health Guard Research Center (H
Talanta ; 224: 121921, 2021 Mar 01.
Article en En | MEDLINE | ID: mdl-33379122
Highly sensitive and accurate measurements of protein biomarkers are crucial for early diagnosis and disease monitoring. Here we report a versatile detection platform for sensitive detection of a protein biomarker using a tandem repeat Spinach aptamer DNA-based transcription immunoassay, which is a immunoassay combined with transcription-assisted Spinach RNA aptamer generation. We designed a DNA template encoding spa tandem repetitive Spinach sequence for enhanced generation of an RNA aptamer. The tandem repeated Spinach DNA template is consist of multiple monomeric units which is composed of T7 promoter, Spinach-2 and terminator. After in vitro transcription, the fluorescence signal from the 16R (nR, n = number of repeats) DNA template was enhanced up to ~ 15-fold compared to a single form (1R) DNA template. Using tandem repeat DNA, the proposed transcription immunoassay showed a limit of detection (LOD) of 37 aM, which is 103-fold lower than that of the conventional enzyme-linked immunosorbent assay (ELISA). The results demonstrate substantial promise for the ultrasensitive detection of various biological analytes using simple ELISA techniques. The high sensitivity and reliability of the proposed transcription immunoassay offer great promise for clinical assays.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aptámeros de Nucleótidos Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Talanta Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aptámeros de Nucleótidos Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Talanta Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos