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Identification of microorganisms directly from blood culture bottles with polymicrobial growth: comparison of FilmArray and direct MALDI-TOF MS.
Almuhayawi, Mohammed S; Wong, Alicia Y W; Kynning, Max; Lüthje, Petra; Ullberg, Måns; Özenci, Volkan.
Afiliación
  • Almuhayawi MS; Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Wong AYW; Department of Microbiology and Medical Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Kynning M; Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Lüthje P; Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Ullberg M; Staatliches Tierärztliches Untersuchungsamt (STUA) Aulendorf - Diagnostikzentrum, Aulendorf, Germany.
  • Özenci V; Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
APMIS ; 129(4): 178-185, 2021 Apr.
Article en En | MEDLINE | ID: mdl-33368673
Bloodstream infections (BSIs) are related to high mortality and morbidity. Rapid administration of effective antimicrobial treatment is crucial for patient survival. Recently developed rapid methods to identify pathogens directly from blood culture bottles speed up diagnosis of BSIs. The present study compares the performance of two rapid identification methods, FilmArray and direct MALDI-TOF MS, on identifying microorganisms directly from positive blood culture bottles with polymicrobial growth. FilmArray and direct MALDI-TOF MS were performed directly on positive clinical and simulated polymicrobial blood culture bottles. Assay results were compared with standard culture methods. In total, 110 polymicrobial blood culture samples, of which 96 samples contained two microorganisms while 14 samples contained three microorganisms, were studied. FilmArray was able to identify 215/234 (92.0%) of isolates detected by the standard culture method and successfully identified all microorganisms in 88/110 (80.0%) of blood culture bottles. In contrast, direct MALDI-TOF MS was only able to identify 65/234 (27.8%) of isolates and managed to identify all microoganisms in 2/110 (2.1%) of blood culture bottles. FilmArray is a rapid method for direct identification of polymicrobial blood culture samples that can complement the conventional identification methods. Direct MALDI-TOF MS has low performance with polymicrobial samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena de la Polimerasa / Sepsis / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Cultivo de Sangre Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: APMIS Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / PATOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Dinamarca
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena de la Polimerasa / Sepsis / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Cultivo de Sangre Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: APMIS Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / PATOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Dinamarca