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Catechin Photolysis Suppression by Aluminum Chloride under Alkaline Conditions and Assessment with Liquid Chromatography-Mass Spectrometry.
Yang, Meei-Ju; Lee, Shwu-Yuan; Liu, Chieh-I; Chen, Shih-Hsuan; Chen, Iou-Zen; Su, Tsung-Chen; Yuann, Jeu-Ming P; Cheng, Chien-Wei; Huang, Shiuh-Tsuen; Liang, Ji-Yuan.
Afiliación
  • Yang MJ; Tea Research and Extension Station, Taoyuan 32654, Taiwan.
  • Lee SY; Department of Tourism and Leisure, Hsing-Wu University, New Taipei City 24452, Taiwan.
  • Liu CI; Department of Biotechnology, Ming-Chuan University, Gui-Shan 33343, Taiwan.
  • Chen SH; Department of Biotechnology, Ming-Chuan University, Gui-Shan 33343, Taiwan.
  • Chen IZ; Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei 10617, Taiwan.
  • Su TC; Tea Research and Extension Station, Taoyuan 32654, Taiwan.
  • Yuann JP; Department of Biotechnology, Ming-Chuan University, Gui-Shan 33343, Taiwan.
  • Cheng CW; Department of Biotechnology, Ming-Chuan University, Gui-Shan 33343, Taiwan.
  • Huang ST; Department of Science Education and Application, National Taichung University of Education, Taichung 40306, Taiwan.
  • Liang JY; Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 40200, Taiwan.
Molecules ; 25(24)2020 Dec 17.
Article en En | MEDLINE | ID: mdl-33348758
Tea is rich in catechins and aluminum. In this study, the process of catechin photolysis was applied as a model for examining the effects of aluminum chloride (AlCl3) on the structural changes of catechin and the alteration of aluminum complexes under blue light irradiation (BLI) at pH 8 using liquid chromatography and mass spectrometry techniques. Additionally, the effects of anions on catechin upon the addition of AlCl3 and treatment with BLI were also studied. In this study, when 1 mM catechin was treated with BLI, a superoxide anion radical (O2•-) was generated in an air-saturated aqueous solution, in addition to forming a dimeric catechin (proanthocyanidin) via a photon-induced redox reaction. The relative percentage of catechin was found to be 59.0 and 95.7 for catechin treated with BLI and catechin upon the addition of 1 mM AlCl3 treated with BLI, respectively. It suggested that catechin treated with BLI could be suppressed by AlCl3, while AlCl3 did not form a complex with catechin in the photolytic system. However, under the same conditions, it was also found that the addition of AlCl3 inhibited the photolytic formation of O2•-, and reduced the generation of proanthocyanidin, suggesting that the disconnection of proanthocyanidin was achieved by AlCl3 acting as a catalyst under treatment with BLI. The influence of 1 mM fluoride (F-) and 1 mM oxalate (C2O42-) ions on the photolysis of 1 mM catechin upon the addition of 1 mM AlCl3 and treatment with BLI was found to be insignificant, implying that, during the photolysis of catechin, the Al species were either neutral or negatively charged and the aluminum species did not form a complex with anions in the photolytic system. Therefore, aluminum, which is an amphoteric species, has an inherent potential to stabilize the photolysis of catechin in an alkaline conditions, while suppressing the O2•- and proanthocyanidin generation via aluminum ion catalysis in the catechin/Al system under treatment with BLI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotólisis / Catequina / Superóxidos / Proantocianidinas / Cloruro de Aluminio Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotólisis / Catequina / Superóxidos / Proantocianidinas / Cloruro de Aluminio Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza