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Physiological electric fields induce directional migration of mammalian cranial neural crest cells.
Mehta, Abijeet Singh; Ha, Pin; Zhu, Kan; Li, ShiYu; Ting, Kang; Soo, Chia; Zhang, Xinli; Zhao, Min.
Afiliación
  • Mehta AS; Department of Ophthalmology & Vision Science, Institute for Regenerative Cures, Center for Neuroscience, University of California at Davis, School of Medicine, Suite 1630, Room 1617, 2921 Stockton Blvd., Sacramento, CA, 95817, USA; Department of Dermatology, University of California, Davis, CA,
  • Ha P; Section of Orthodontics, Division of Growth and Development, School of Dentistry, University of California, Los Angeles, CA, USA.
  • Zhu K; Department of Ophthalmology & Vision Science, Institute for Regenerative Cures, Center for Neuroscience, University of California at Davis, School of Medicine, Suite 1630, Room 1617, 2921 Stockton Blvd., Sacramento, CA, 95817, USA; Department of Dermatology, University of California, Davis, CA,
  • Li S; Department of Ophthalmology & Vision Science, Institute for Regenerative Cures, Center for Neuroscience, University of California at Davis, School of Medicine, Suite 1630, Room 1617, 2921 Stockton Blvd., Sacramento, CA, 95817, USA; Department of Dermatology, University of California, Davis, CA,
  • Ting K; Section of Orthodontics, Division of Growth and Development, School of Dentistry, University of California, Los Angeles, CA, USA.
  • Soo C; Division of Plastic and Reconstructive Surgery and Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, University of California, Los Angeles, CA, 90095, USA.
  • Zhang X; Section of Orthodontics, Division of Growth and Development, School of Dentistry, University of California, Los Angeles, CA, USA. Electronic address: xzhang@dentistry.ucla.edu.
  • Zhao M; Department of Ophthalmology & Vision Science, Institute for Regenerative Cures, Center for Neuroscience, University of California at Davis, School of Medicine, Suite 1630, Room 1617, 2921 Stockton Blvd., Sacramento, CA, 95817, USA; Department of Dermatology, University of California, Davis, CA,
Dev Biol ; 471: 97-105, 2021 03.
Article en En | MEDLINE | ID: mdl-33340512
During neurulation, cranial neural crest cells (CNCCs) migrate long distances from the neural tube to their terminal site of differentiation. The pathway traveled by the CNCCs defines the blueprint for craniofacial construction, abnormalities of which contribute to three-quarters of human birth defects. Biophysical cues like naturally occurring electric fields (EFs) have been proposed to be one of the guiding mechanisms for CNCC migration from the neural tube to identified position in the branchial arches. Such endogenous EFs can be mimicked by applied EFs of physiological strength that has been reported to guide the migration of amphibian and avian neural crest cells (NCCs), namely galvanotaxis or electrotaxis. However, the behavior of mammalian NCCs in external EFs has not been reported. We show here that mammalian CNCCs migrate towards the anode in direct current (dc) EFs. Reversal of the field polarity reverses the directedness. The response threshold was below 30 â€‹mV/mm and the migration directedness and displacement speed increased with increase in field strength. Both CNCC line (O9-1) and primary mouse CNCCs show similar galvanotaxis behavior. Our results demonstrate for the first time that the mammalian CNCCs respond to physiological EFs by robust directional migration towards the anode in a voltage-dependent manner.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Región Branquial / Transducción de Señal / Diferenciación Celular / Movimiento Celular / Electricidad Límite: Animals Idioma: En Revista: Dev Biol Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Región Branquial / Transducción de Señal / Diferenciación Celular / Movimiento Celular / Electricidad Límite: Animals Idioma: En Revista: Dev Biol Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos