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IMXQB-80: A Quillaja brasiliensis saponin-based nanoadjuvant enhances Zika virus specific immune responses in mice.
Cibulski, Samuel; Teixeira, Thais Fumaco; Varela, Ana Paula Muterle; de Lima, Matheus Fabião; Casanova, Gabriela; Nascimento, Yuri Mangueira; Fechine Tavares, Josean; da Silva, Marcelo Sobral; Sesterheim, Patrícia; Souza, Diogo Onofre; Roehe, Paulo Michel; Silveira, Fernando.
Afiliación
  • Cibulski S; Centro de Biotecnologia - CBiotec, Laboratório de Biotecnologia Celular e Molecular, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil; Departamento de Microbiologia Imunologia e Parasitologia, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Porto Alegr
  • Teixeira TF; Departamento de Microbiologia Imunologia e Parasitologia, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
  • Varela APM; Departamento de Microbiologia Imunologia e Parasitologia, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
  • de Lima MF; Centro de Biotecnologia - CBiotec, Laboratório de Biotecnologia Celular e Molecular, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil.
  • Casanova G; Unidad de Microscopía Electrónica de Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
  • Nascimento YM; Institute for Research in Pharmaceutical and Medications, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil.
  • Fechine Tavares J; Institute for Research in Pharmaceutical and Medications, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil.
  • da Silva MS; Institute for Research in Pharmaceutical and Medications, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil.
  • Sesterheim P; Centro de Cardiologia Experimental, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Porto Alegre, RS, Brazil.
  • Souza DO; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Roehe PM; Departamento de Microbiologia Imunologia e Parasitologia, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
  • Silveira F; Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República (UdelaR), Montevideo, Uruguay. Electronic address: fernandosilveiragonzalez@gmail.com.
Vaccine ; 39(3): 571-579, 2021 01 15.
Article en En | MEDLINE | ID: mdl-33339669
Vaccine adjuvants are compounds that enhance/prolong the immune response to a co-administered antigen. Saponins have been widely used as adjuvants for many years in several vaccines - especially for intracellular pathogens - including the recent and somewhat revolutionary malaria and shingles vaccines. In view of the immunoadjuvant potential of Q. brasiliensis saponins, the present study aimed to characterize the QB-80 saponin-rich fraction and a nanoadjuvant prepared with QB-80 and lipids (IMXQB-80). In addition, the performance of such adjuvants was examined in experimental inactivated vaccines against Zika virus (ZIKV). Analysis of QB-80 by DI-ESI-ToF by negative ion electrospray revealed over 29 saponins that could be assigned to known structures existing in their congener Q. saponaria, including the well-studied QS-21 and QS-7. The QB-80 saponins were a micrOTOF able to self-assembly with lipids in ISCOM-like nanoparticles with diameters of approximately 43 nm, here named IMXQB-80. Toxicity assays revealed that QB-80 saponins did present some haemolytical and cytotoxic potentials; however, these were abrogated in IMXQB-80 nanoparticles. Regarding the adjuvant activity, QB-80 and IMXQB-80 significantly enhanced serum levels of anti-Zika virus IgG and subtypes (IgG1, IgG2b, IgG2c) as well as neutralized antibodies when compared to an unadjuvanted vaccine. Furthermore, the nanoadjuvant IMXQB-80 was as effective as QB-80 in stimulating immune responses, yet requiring fourfold less saponins to induce the equivalent stimuli, and with less toxicity. These findings reveal that the saponin fraction QB-80, and particularly the IMXQB-80 nanoadjuvant, are safe and capable of potentializing immune responses when used as adjuvants in experimental ZIKV vaccines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saponinas / Virus Zika / Infección por el Virus Zika Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Vaccine Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saponinas / Virus Zika / Infección por el Virus Zika Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Vaccine Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos