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miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia-archival samples study.
Pelka, Kacper; Klicka, Klaudia; Grzywa, Tomasz M; Gondek, Agata; Marczewska, Janina M; Garbicz, Filip; Szczepaniak, Kinga; Paskal, Wiktor; Wlodarski, Pawel K.
Afiliación
  • Pelka K; The Department of Methodology, Center for Preclinical Research, Medical University of Warsaw, 1B Banacha Street, 02-097, Warsaw, Poland.
  • Klicka K; The Department of Methodology, Center for Preclinical Research, Medical University of Warsaw, 1B Banacha Street, 02-097, Warsaw, Poland.
  • Grzywa TM; Doctoral School, Medical University of Warsaw, 61 Zwirki i Wigury Street, 02-091, Warsaw, Poland.
  • Gondek A; The Department of Methodology, Center for Preclinical Research, Medical University of Warsaw, 1B Banacha Street, 02-097, Warsaw, Poland.
  • Marczewska JM; Doctoral School, Medical University of Warsaw, 61 Zwirki i Wigury Street, 02-091, Warsaw, Poland.
  • Garbicz F; Department of Immunology, Medical University of Warsaw, 5 Nielubowicza Street, 02-097, Warsaw, Poland.
  • Szczepaniak K; The Department of Methodology, Center for Preclinical Research, Medical University of Warsaw, 1B Banacha Street, 02-097, Warsaw, Poland.
  • Paskal W; The Department of Pathology, Medical University of Warsaw, 7 Pawinskiego Street, 02-106, Warsaw, Poland.
  • Wlodarski PK; The Department of Methodology, Center for Preclinical Research, Medical University of Warsaw, 1B Banacha Street, 02-097, Warsaw, Poland.
Histochem Cell Biol ; 155(3): 423-433, 2021 Mar.
Article en En | MEDLINE | ID: mdl-33331954
MicroRNAs are involved in various pathologies including cancer. The aim of the study was to assess the level of expression of miR-96-5p, -134-5p, -181b-5p, -200b-3p in FFPE samples of prostate cancer, adjacent cancer-free tissue, and benign prostatic hyperplasia. Samples of 23 FFPE prostate cancer and 22 benign prostatic hyperplasias were dissected and HE stained. Compartments of tumor tissue and adjacent healthy glandular tissue were isolated from each sample using Laser Capture Microdissection. Total RNA was isolated from dissected tissues. Expression of miR-96-5p, miR-134-5p, 181b-5p, and miR-200b-3p was determined by real-time RT-qPCR method. The expression of miR-200b-3p was significantly higher in cancerous prostate: both in adenocarcinomatous glands and in the adjacent, apparently unaffected glands compared to BPH samples. The expression of miR-181b-5p was lower in in both prostate cancer tissues and adjacent tissue compared to BPH samples. Expression of miR-96-5p and miR-134-5p was lower in prostate cancer tissues compared to BPH. Levels of miR-96-5p, miR-134-5p, and 181b-5p negatively correlated with the Gleason score. Given further studies, miR-96-5p, miR-134-5p and especially miR-200b-3p and miR-181b-5p may differentiate BPH and PC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hiperplasia Prostática / Neoplasias de la Próstata / MicroARNs Límite: Aged / Humans / Male / Middle aged Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hiperplasia Prostática / Neoplasias de la Próstata / MicroARNs Límite: Aged / Humans / Male / Middle aged Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania