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An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A.
Ciriaco, Fulvio; De Leo, Vincenzo; Catucci, Lucia; Pascale, Michelangelo; Logrieco, Antonio F; DeRosa, Maria C; De Girolamo, Annalisa.
Afiliación
  • Ciriaco F; Department of Chemistry, University of Bari, Via Orabona 4, 70126 Bari, Italy.
  • De Leo V; Department of Chemistry, University of Bari, Via Orabona 4, 70126 Bari, Italy.
  • Catucci L; Department of Chemistry, University of Bari, Via Orabona 4, 70126 Bari, Italy.
  • Pascale M; Institute of Sciences of Food Production (ISPA), CNR-National Research Council of Italy, Via G. Amendola 122/O, 70126 Bari, Italy.
  • Logrieco AF; Institute of Sciences of Food Production (ISPA), CNR-National Research Council of Italy, Via G. Amendola 122/O, 70126 Bari, Italy.
  • DeRosa MC; Department of Chemistry, Carleton University, 1125 Colonel by Drive, Ottawa, ON K1S 5B6, Canada.
  • De Girolamo A; Institute of Sciences of Food Production (ISPA), CNR-National Research Council of Italy, Via G. Amendola 122/O, 70126 Bari, Italy.
Polymers (Basel) ; 12(12)2020 Dec 14.
Article en En | MEDLINE | ID: mdl-33327526
Aptamers are single-stranded oligonucleotides selected by SELEX (Systematic Evolution of Ligands by EXponential Enrichment) able to discriminate target molecules with high affinity and specificity, even in the case of very closely related structures. Aptamers have been produced for several targets including small molecules like mycotoxins; however, the high affinity for their respective target molecules is a critical requirement. In the last decade, the screening through computational methods of aptamers for their affinity against specific targets has greatly increased and is becoming a commonly used procedure due to its convenience and low costs. This paper describes an in-silico approach for rapid screening of ten ssDNA aptamer sequences against fumonisin B1 (FB1, n = 3), aflatoxin B1 (AFB1, n = 2) and ochratoxin A (OTA, n = 5). Theoretical results were compared with those obtained by testing the same aptamers by fluorescent microscale thermophoresis and by magnetic beads assay for their binding affinity (KD) revealing a good agreement.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Polymers (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Polymers (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza