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CNS macrophages differentially rely on an intronic Csf1r enhancer for their development.
Munro, David A D; Bradford, Barry M; Mariani, Samanta A; Hampton, David W; Vink, Chris S; Chandran, Siddharthan; Hume, David A; Pridans, Clare; Priller, Josef.
Afiliación
  • Munro DAD; UK Dementia Research Institute at The University of Edinburgh, Chancellor's Building, Edinburgh EH16 4SB, UK.
  • Bradford BM; The Roslin Institute & Royal (Dick) School of Veterinary Sciences, The University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
  • Mariani SA; Centre for Inflammation Research, Queen's Medical Research Institute, The University of Edinburgh, Edinburgh EH16 4TJ, UK.
  • Hampton DW; Euan MacDonald Centre for MND Research, The University of Edinburgh, Edinburgh EH16 4SB, UK.
  • Vink CS; Centre for Clinical Brain Sciences, The University of Edinburgh, Edinburgh EH16 4SB, UK.
  • Chandran S; Centre for Inflammation Research, Queen's Medical Research Institute, The University of Edinburgh, Edinburgh EH16 4TJ, UK.
  • Hume DA; UK Dementia Research Institute at The University of Edinburgh, Chancellor's Building, Edinburgh EH16 4SB, UK.
  • Pridans C; Euan MacDonald Centre for MND Research, The University of Edinburgh, Edinburgh EH16 4SB, UK.
  • Priller J; Centre for Clinical Brain Sciences, The University of Edinburgh, Edinburgh EH16 4SB, UK.
Development ; 147(23)2020 12 15.
Article en En | MEDLINE | ID: mdl-33323375
The central nervous system hosts parenchymal macrophages, known as microglia, and non-parenchymal macrophages, collectively termed border-associated macrophages (BAMs). Microglia, but not BAMs, were reported to be absent in mice lacking a conserved Csf1r enhancer: the fms-intronic regulatory element (FIRE). However, it is unknown whether FIRE deficiency also impacts BAM arrival and/or maintenance. Here, we show that macrophages in the ventricular system of the brain, including Kolmer's epiplexus macrophages, are absent in Csf1rΔFIRE/ΔFIRE mice. Stromal choroid plexus BAMs are also considerably reduced. During normal development, we demonstrate that intracerebroventricular macrophages arrive from embryonic day 10.5, and can traverse ventricular walls in embryonic slice cultures. In Csf1rΔFIRE/ΔFIRE embryos, the arrival of both primitive microglia and intracerebroventricular macrophages was eliminated, whereas the arrival of cephalic mesenchyme and stromal choroid plexus BAMs was only partially restricted. Our results provide new insights into the development and regulation of different CNS macrophage populations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elementos de Facilitación Genéticos / Receptores de Factor Estimulante de Colonias de Granulocitos y Macrófagos / Desarrollo Embrionario / Macrófagos Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elementos de Facilitación Genéticos / Receptores de Factor Estimulante de Colonias de Granulocitos y Macrófagos / Desarrollo Embrionario / Macrófagos Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido