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Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions.
Pérez-Ramírez, Elisa; Cano-Gómez, Cristina; Llorente, Francisco; Vodica, Ani; Veljovic, Ljubisa; Toklikishvilli, Natela; Sherifi, Kurtesh; Sghaier, Soufien; Omani, Amel; Kustura, Aida; Krstevski, Kiril; Karayel-Hacioglu, Ilke; Hagag, Naglaa Mohamed; El Hage, Jeanne; Davdyan, Hasmik; Bintarif, Mohd Saddam; Adzic, Bojan; Abouchoaib, Nabil; Jiménez-Clavero, Miguel Ángel; Fernández-Pinero, Jovita.
Afiliación
  • Pérez-Ramírez E; Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CISA), 28130 Valdeolmos, Spain.
  • Cano-Gómez C; Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CISA), 28130 Valdeolmos, Spain.
  • Llorente F; Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CISA), 28130 Valdeolmos, Spain.
  • Vodica A; Department of Animal Health, Food Safety and Veterinary Institute, Tirana, Albania.
  • Veljovic L; Virology Department, Scientific Institute of Veterinary Medicine of Serbia, 11000 Belgrade, Serbia.
  • Toklikishvilli N; Laboratory of Virology and Molecular Biology, LEPL State Laboratory of Agriculture (SLA), 0159 Tbilisi, Georgia.
  • Sherifi K; Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Sciences, University of Prishtina "Hasan Pristhina", 10000 Prishtine, Kosovo.
  • Sghaier S; Virology Department, Institute of Veterinary Research of Tunisia, 1006 Tunis, Tunisia.
  • Omani A; Laboratoire Central Vétérinaire d'Alger, Institut National de la Médecine Vétérinaire, Algiers, Algeria.
  • Kustura A; Veterinary Faculty, University of Sarajevo, 71000 Sarajevo, Bosnia and Herzegovina.
  • Krstevski K; Faculty of Veterinary Medicine, Ss. Cyril and Methodius University, 1000 Skopje, North Macedonia.
  • Karayel-Hacioglu I; Virology Department, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey.
  • Hagag NM; Animal Health Research Institute, Dokki 12618, Egypt.
  • El Hage J; Animal Health Laboratory, Lebanese Agricultural Research Institute, 90-1064 Fanar, Lebanon.
  • Davdyan H; Republican Veterinary-Sanitary and Phytosanitary Center of Laboratory Services SNCO, Yerevan, Armenia.
  • Bintarif MS; Animal Wealth Laboratory Sector, Ministry of Agriculture, Amman, Jordan.
  • Adzic B; Diagnostic Veterinary Laboratory, 81000 Podgorica, Montenegro.
  • Abouchoaib N; Casablanca Regional Research and Analysis Laboratory of National Office of Sanitary Safety and Food Products (ONSSA), Nouaceur, 20 000 Casablanca, Morocco.
  • Jiménez-Clavero MÁ; Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CISA), 28130 Valdeolmos, Spain.
  • Fernández-Pinero J; CIBER Epidemiología y Salud Pública (CIBERESP), 28029 Madrid, Spain.
Pathogens ; 9(12)2020 Dec 11.
Article en En | MEDLINE | ID: mdl-33322276
The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Pathogens Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Pathogens Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza