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Quantitative Determination of Acrolein in Cider by 1H NMR Spectrometry.
de Las Heras, Enaitz; Zuriarrain-Ocio, Andoni; Zuriarrain, Juan; Bordagaray, Ane; Dueñas, María Teresa; Berregi, Iñaki.
Afiliación
  • de Las Heras E; Faculty of Chemistry, University of the Basque Country EHU/UPV, 20018 Donostia-San Sebastián, Spain.
  • Zuriarrain-Ocio A; Faculty of Chemistry, University of the Basque Country EHU/UPV, 20018 Donostia-San Sebastián, Spain.
  • Zuriarrain J; Faculty of Chemistry, University of the Basque Country EHU/UPV, 20018 Donostia-San Sebastián, Spain.
  • Bordagaray A; Faculty of Chemistry, University of the Basque Country EHU/UPV, 20018 Donostia-San Sebastián, Spain.
  • Dueñas MT; Faculty of Chemistry, University of the Basque Country EHU/UPV, 20018 Donostia-San Sebastián, Spain.
  • Berregi I; Faculty of Chemistry, University of the Basque Country EHU/UPV, 20018 Donostia-San Sebastián, Spain.
Foods ; 9(12)2020 Dec 08.
Article en En | MEDLINE | ID: mdl-33302379
Acrolein occasionally appears in cider, completely spoiling its quality due to its bitter taste. It is crucial to detect it in the early steps, before the taste is severely affected, to apply the appropriate treatment. A simple and rapid analytical method to determine this compound in cider is therefore desirable. In this work, a quantitative determination method of acrolein in cider is proposed using the proton nuclear magnetic resonance technique (1H NMR). Acrolein produces a doublet signal in the spectrum at 9.49 ppm, whose area is used to determine the concentration of this compound. Importantly, 3-(Trimethylsilyl)-2,2,3,3-d4-propionic acid sodium salt is added to the cider as a reference for 0.00 ppm and 1,3,5-benzenetricarboxylic acid as an internal standard for acrolein determination. The method is validated by gas chromatography (GC). There is a good correlation between the acrolein concentrations obtained by 1H NMR and by gas chromatography in different commercial ciders (Pearson coefficient 0.9994). The 95% confidence interval for the intercept is 0.15 ± 0.49 (includes 0) and for the slope is 0.98 ± 0.03 (includes 1). When applying the paired t test, no significant difference is observed. The proposed method is direct, and no prior derivatization is needed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza