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Precision targeting of bacterial pathogen via bi-functional nanozyme activated by biofilm microenvironment.
Huang, Yue; Liu, Yuan; Shah, Shrey; Kim, Dongyeop; Simon-Soro, Aurea; Ito, Tatsuro; Hajfathalian, Maryam; Li, Yong; Hsu, Jessica C; Nieves, Lenitza M; Alawi, Faizan; Naha, Pratap C; Cormode, David P; Koo, Hyun.
Afiliación
  • Huang Y; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States; Biofilm Research Labs, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States; Department of Orthodontics and Divisio
  • Liu Y; Biofilm Research Labs, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States; Department of Orthodontics and Divisions of Pediatric Dentistry & Community Oral Health, School of Dental Medicine, University of Pennsylvania, Phila
  • Shah S; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States; Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA, 19104, United States.
  • Kim D; Biofilm Research Labs, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States; Department of Orthodontics and Divisions of Pediatric Dentistry & Community Oral Health, School of Dental Medicine, University of Pennsylvania, Phila
  • Simon-Soro A; Biofilm Research Labs, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States; Department of Orthodontics and Divisions of Pediatric Dentistry & Community Oral Health, School of Dental Medicine, University of Pennsylvania, Phila
  • Ito T; Biofilm Research Labs, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States; Department of Orthodontics and Divisions of Pediatric Dentistry & Community Oral Health, School of Dental Medicine, University of Pennsylvania, Phila
  • Hajfathalian M; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
  • Li Y; Biofilm Research Labs, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States.
  • Hsu JC; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States; Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA, 19104, United States.
  • Nieves LM; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
  • Alawi F; Department of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19014, United States.
  • Naha PC; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
  • Cormode DP; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States; Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA, 19104, United States; Department of Cardiology, University of Pen
  • Koo H; Biofilm Research Labs, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, United States; Department of Orthodontics and Divisions of Pediatric Dentistry & Community Oral Health, School of Dental Medicine, University of Pennsylvania, Phila
Biomaterials ; 268: 120581, 2021 01.
Article en En | MEDLINE | ID: mdl-33302119
Human dental caries is an intractable biofilm-associated disease caused by microbial interactions and dietary sugars on the host's teeth. Commensal bacteria help control opportunistic pathogens via bioactive products such as hydrogen peroxide (H2O2). However, high-sugar consumption disrupts homeostasis and promotes pathogen accumulation in acidic biofilms that cause tooth-decay. Here, we exploit the pathological (sugar-rich/acidic) conditions using a nanohybrid system to increase intrinsic H2O2 production and trigger pH-dependent reactive oxygen species (ROS) generation for efficient biofilm virulence targeting. The nanohybrid contains glucose-oxidase that catalyzes glucose present in biofilms to increase intrinsic H2O2, which is converted by iron oxide nanoparticles with peroxidase-like activity into ROS in acidic pH. Notably, it selectively kills Streptococcus mutans (pathogen) without affecting Streptococcus oralis (commensal) via preferential pathogen-binding and in situ ROS generation. Furthermore, nanohybrid treatments potently reduced dental caries in a rodent model. Compared to chlorhexidine (positive-control), which disrupted oral microbiota diversity, the nanohybrid had significant higher efficacy without affecting soft-tissues and the oral-gastrointestinal microbiomes, while modulating dental health-associated microbial activity in vivo. The data reveal therapeutic precision of a bi-functional hybrid nanozyme against a biofilm-related disease in a controlled-manner activated by pathological conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caries Dental / Peróxido de Hidrógeno Límite: Humans Idioma: En Revista: Biomaterials Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caries Dental / Peróxido de Hidrógeno Límite: Humans Idioma: En Revista: Biomaterials Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos