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Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria.
Schneider Werner Vianna, Thiago; Sartoretto, Suelen Cristina; Neves Novellino Alves, Adriana Terezinha; Figueiredo de Brito Resende, Rodrigo; de Almeida Barros Mourão, Carlos Fernando; de Albuquerque Calasans-Maia, Jose; Martinez-Zelaya, Victor R; Malta Rossi, Alexandre; Granjeiro, Jose Mauro; Calasans-Maia, Monica Diuana; Seabra Louro, Rafael.
Afiliación
  • Schneider Werner Vianna T; Pos-Graduation Program, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.
  • Sartoretto SC; Oral Surgery Department, Universidade Veiga de Almeida, Rio de Janeiro 20271-020, Brazil.
  • Neves Novellino Alves AT; Oral Surgery Department, Universidade Iguaçu, Rio de Janeiro 26260-045, Brazil.
  • Figueiredo de Brito Resende R; Oral Diagnosis Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.
  • de Almeida Barros Mourão CF; Oral Surgery Department, Universidade Iguaçu, Rio de Janeiro 26260-045, Brazil.
  • de Albuquerque Calasans-Maia J; Oral Surgery Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.
  • Martinez-Zelaya VR; Pos-Graduation Program, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.
  • Malta Rossi A; Orthodontic Department, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.
  • Granjeiro JM; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), São Paulo 13083-970, Brazil.
  • Calasans-Maia MD; Department of Condensed Matter, Applied Physics and Nanoscience, Brazilian Center for Research in Physics, Rio de Janeiro 22290-180, Brazil.
  • Seabra Louro R; Pos-Graduation Program, Dentistry School, Universidade Federal Fluminense, Rio de Janeiro 24020-140, Brazil.
J Funct Biomater ; 11(4)2020 Dec 04.
Article en En | MEDLINE | ID: mdl-33291525
Many biomaterials are used for Bone Morphogenetic Proteins (BMPs) delivery in bone tissue engineering. The BMP carrier system's primary function is to hold these growth factors at the wound's site for a prolonged time and provide initial support for cells to attach and elaborate the extracellular matrix for bone regeneration. This study aimed to evaluate the nanostructured carbonated hydroxyapatite microspheres (nCHA) as an rhBMP-2 carrier on rats calvaria. A total of fifteen male Wistar rats were randomly divided into three groups (n = 5): clot (control group), rhBMP-2 associated with collagen membrane (COL/rhBMP-2) or associated with the microspheres (nCHA/rhBMP-2). After 45 days, the calvaria defect samples were evaluated through histological, histomorphometric, and SR-µCT analyses to investigate new-formed bone and connective tissue volume densities. The descriptive histological analysis showed that nCHA/rhBMP-2 improved bone formation compared to other groups. These results were confirmed by histomorphometric and SR-µCT analysis that showed substantially defect area filling with a higher percentage of newly formed (36.24 ± 6.68) bone than those with the COL/rhBMP-2 (0.42 ± 0.40) and Clot (3.84 ± 4.57) (p < 0.05). The results showed that nCHA is an effective carrier for rhBMP-2 encouraging bone healing and an efficient alternative to collagen membrane for rhBMP-2 delivery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: J Funct Biomater Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: J Funct Biomater Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza