Your browser doesn't support javascript.
loading
High throughput acoustic microfluidic mixer controls self-assembly of protein nanoparticles with tuneable sizes.
Pourabed, Amir; Younas, Tayyaba; Liu, Chang; Shanbhag, Bhuvana K; He, Lizhong; Alan, Tuncay.
Afiliación
  • Pourabed A; Department of Mechanical and Aerospace Engineering, Monash University, Wellington Road, Clayton 3800, Australia.
  • Younas T; Department of Chemical Engineering, Monash University, Wellington Road, Clayton 3800, Australia.
  • Liu C; Department of Chemical Engineering, Monash University, Wellington Road, Clayton 3800, Australia.
  • Shanbhag BK; Department of Chemical Engineering, Monash University, Wellington Road, Clayton 3800, Australia.
  • He L; Department of Chemical Engineering, Monash University, Wellington Road, Clayton 3800, Australia. Electronic address: Lizhong.he@monash.edu.
  • Alan T; Department of Mechanical and Aerospace Engineering, Monash University, Wellington Road, Clayton 3800, Australia. Electronic address: Tuncay.alan@monash.edu.
J Colloid Interface Sci ; 585: 229-236, 2021 Mar.
Article en En | MEDLINE | ID: mdl-33285461
HYPOTHESIS: Protein nanoparticles have attracted increased interest due to their broad applications ranging from drug delivery and vaccines to biocatalysts and biosensors. The morphology and the size of the nanoparticles play a crucial role in determining their suitability for different applications. Yet, effectively controlling the size of the nanoparticles is still a significant challenge in their manufacture. The hypothesis of this paper is that the assembly conditions and size of protein particles can be tuned via a mechanical route by simply modifying the mixing time and strength, while keeping the chemical parameters constant. EXPERIMENTAL: We use an acoustically actuated, high throughput, ultrafast, microfluidic mixer for the assembly of protein particles with tuneable sizes. The performance of the acoustic micro-mixer is characterized via Laser Doppler Vibrometry and image processing. The assembly of protein nanoparticles is monitored by dynamic light scattering (DLS) and transmission electron microscopy (TEM). FINDINGS: By changing actuation parameters, the turbulence and mixing in the microchannel can be precisely varied to control the initiation of protein particle assembly while the solution conditions of assembly (pH and ionic strength) are kept constant. Importantly, mixing times as low as 6 ms can be achieved for triggering protein assembly in the microfluidic channel. In comparison to the conventional batch process of assembly, the acoustic microfluidic mixer approach produces smaller particles with a more uniform size distribution, promising a new way to manufacture protein particles with controllable quality.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Colloid Interface Sci Año: 2021 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Colloid Interface Sci Año: 2021 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos