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Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices.
Belle, Mino D C; Baño-Otalora, Beatriz; Piggins, Hugh D.
Afiliación
  • Belle MDC; Faculty of Biology, Medicine, and Health, AV Hill Building, University of Manchester, Manchester, UK. m.d.c.belle@exeter.ac.uk.
  • Baño-Otalora B; Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exeter, UK. m.d.c.belle@exeter.ac.uk.
  • Piggins HD; Faculty of Biology, Medicine, and Health, AV Hill Building, University of Manchester, Manchester, UK.
Methods Mol Biol ; 2130: 263-285, 2021.
Article en En | MEDLINE | ID: mdl-33284451
The ability to record ensemble action potential (AP) discharge frequencies from large populations of neurons over extended periods of time in vitro offers clear advantages in neuroscience and circadian biology research. Here, we provide an overview of a step-by-step method to perform multisite extracellular AP activity recordings in suprachiasmatic and mediobasal hypothalamic nuclei brain slices, using a state-of-the-art perforated multielectrode array system. Further, we describe in detail a setup architecture which systematically delivers stable, high-quality recordings with excellent anatomical accuracy and consistency. We also provide some procedural, technical, and methodological troubleshooting notes and examples of good quality recordings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Supraquiasmático / Potenciales de Acción / Electroencefalografía Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Supraquiasmático / Potenciales de Acción / Electroencefalografía Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos