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Common and personal target genes of the micronutrient vitamin D in primary immune cells from human peripheral blood.
Hanel, Andrea; Neme, Antonio; Malinen, Marjo; Hämäläinen, Emmi; Malmberg, Henna-Riikka; Etheve, Stéphane; Tuomainen, Tomi-Pekka; Virtanen, Jyrki K; Bendik, Igor; Carlberg, Carsten.
Afiliación
  • Hanel A; School of Medicine, Institute of Biomedicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland.
  • Neme A; Institute for Applied Mathematics, Merida Research Unit, National Autonomous University of Mexico, Sierra Papacal, Merida, Yucatan, Mexico.
  • Malinen M; Department of Environmental and Biological Sciences, University of Eastern Finland, Joensuu, Finland.
  • Hämäläinen E; School of Medicine, Institute of Biomedicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland.
  • Malmberg HR; School of Medicine, Institute of Biomedicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland.
  • Etheve S; DSM Nutritional Products Inc., R&D Solution Center, Kaiseraugst, Switzerland.
  • Tuomainen TP; Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland.
  • Virtanen JK; Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland.
  • Bendik I; DSM Nutritional Products Inc., Human Nutrition and Health, Kaiseraugst, Switzerland.
  • Carlberg C; School of Medicine, Institute of Biomedicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland. carsten.carlberg@uef.fi.
Sci Rep ; 10(1): 21051, 2020 12 03.
Article en En | MEDLINE | ID: mdl-33273683
Vitamin D is essential for the function of the immune system. In this study, we treated peripheral blood mononuclear cells (PBMCs) of healthy adults with the biologically active form of vitamin D3, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) using two different approaches: single repeats with PBMCs obtained from a cohort of 12 individuals and personalized analysis based on triplicates of five study participants. This identified 877 (cohort approach) and 3951 (personalized approach) genes that significantly (p < 0.05) changed their expression 24 h after 1,25(OH)2D3 stimulation. From these, 333 and 1232 were classified as supertargets, a third of which were identified as novel. Individuals differed largely in their vitamin D response not only by the magnitude of expression change but also by their personal selection of (super)target genes. Functional analysis of the target genes suggested the overarching role of vitamin D in the regulation of metabolism, proliferation and differentiation, but in particular in the control of functions mediated by the innate and adaptive immune system, such as responses to infectious diseases and chronic inflammatory disorders. In conclusion, immune cells are an important target of vitamin D and common genes may serve as biomarkers for personal responses to the micronutrient.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vitamina D / Leucocitos Mononucleares / Micronutrientes Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vitamina D / Leucocitos Mononucleares / Micronutrientes Tipo de estudio: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Reino Unido