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A multi-country field validation of the FluChip-8G Insight Assay.
Velasco, John Mark; Shrestha, Sanjaya; Valderama, Maria Theresa; Shrestha, Jasmin; Shrestha, Binob; Diones, Paula Corazon; Leonardia, Susie; Poolpanichupatam, Yongyuth; Hussem, Kittinun; Chua, Domingo; Navarro, Fatima Claire; Develos, Maribel; Macareo, Louis; Ellison, Damon; Fernandez, Stefan; Klungthong, Chonticha.
Afiliación
  • Velasco JM; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; University of the Philippines Manila, Ermita, Manila, Philippines. Electronic address: JSVelasco@up.edu.ph.
  • Shrestha S; Walter Reed/AFRIMS Research Unit Nepal (WARUN), Kathmandu, Nepal.
  • Valderama MT; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Shrestha J; Walter Reed/AFRIMS Research Unit Nepal (WARUN), Kathmandu, Nepal.
  • Shrestha B; Walter Reed/AFRIMS Research Unit Nepal (WARUN), Kathmandu, Nepal.
  • Diones PC; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Leonardia S; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Poolpanichupatam Y; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Hussem K; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Chua D; V Luna Medical Center, Armed Forces of the Philippines Health Service Command, Quezon City, Philippines.
  • Navarro FC; V Luna Medical Center, Armed Forces of the Philippines Health Service Command, Quezon City, Philippines.
  • Develos M; V Luna Medical Center, Armed Forces of the Philippines Health Service Command, Quezon City, Philippines.
  • Macareo L; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Ellison D; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Fernandez S; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Klungthong C; U.S. Army Medical Directorate - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. Electronic address: ChontichaK.fsn@afrims.org.
J Virol Methods ; 289: 114029, 2021 03.
Article en En | MEDLINE | ID: mdl-33271255
INTRODUCTION: It is critical to rapidly detect novel and non-seasonal influenza strains. Currently available assays have limited sensitivity in detecting novel influenza subtypes. We performed a multi-country field validation of the FluChip-8G Insight, an assay able to detect and characterize influenza A/B viruses and non-seasonal influenza viruses. MATERIALS AND METHODS: We evaluated the performance of the FluChip-8G Insight on nasal and throat swab clinical samples from Thailand, Philippines and Nepal. Influenza PCR positive and negative samples tested using the US CDC Human Influenza Dx Panel reference standard were selected for testing using the FluChip-8G Influenza Insight. RESULTS: A total of 909 specimens were included in the analysis. The overall sensitivity and specificity of the FluChip-8G Insight to detect combined influenza A+B was 86 % and 100%, respectively. PPV and NPV were estimated at 100 % (95 % CI 99-100) and 73 % (95 % CI 68-78), respectively. Sensitivity across all influenza subtypes was 100% for specimens with <20 and 20-25 Ct values, respectively, but as Ct values increased, sensitivity across all influenza subtypes decreased significantly (p < 0.001) for specimens with Ct values ≥32. CONCLUSION: The FluChip-8G Insight showed good precision and reproducibility among all 3 sites with robust identification of both influenza A and B targets with Ct values <32 and in the absence of co-infection. Positioning this platform in countries considered as hotspots for the emergence of novel/zoonotic influenza strains can increase the lead time in detecting and containing novel influenza strains with pandemic potential.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Influenza A / Gripe Humana Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Virol Methods Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Influenza A / Gripe Humana Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Virol Methods Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos