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A gold nanoparticle-based immunochromatographic assay for simultaneous detection of multiplex sildenafil adulterants in health food by only one antibody.
He, Fan; Yang, Jinyi; Zou, Tingting; Xu, Zhenlin; Tian, Yuanxin; Sun, Wenjia; Wang, Hong; Sun, Yuanming; Lei, Hongtao; Chen, Zijian; Liu, Juewen; Tan, Xuecai; Shen, Yudong.
Afiliación
  • He F; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China; Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Yang J; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China.
  • Zou T; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China.
  • Xu Z; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China.
  • Tian Y; Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, Guangdong, PR China. Electronic address: tyx523@163.com.
  • Sun W; Guangdong Provincial Institute of Food Inspection, Guangzhou, 510435, Guangdong, PR China.
  • Wang H; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China.
  • Sun Y; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China.
  • Lei H; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China.
  • Chen Z; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China.
  • Liu J; Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. Electronic address: liujw@uwaterloo.ca.
  • Tan X; College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, 530006, PR China.
  • Shen Y; College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, Guangdong, PR China. Electronic address: shenyudong@scau.edu.cn.
Anal Chim Acta ; 1141: 1-12, 2021 Jan 02.
Article en En | MEDLINE | ID: mdl-33248641
In the last few years, sildenafil and its analogues have emerged as an illegal ingredient in health food and products, causing illness and death. To address this problem, herein, a gold nanoparticle-based immunochromatographic assay for simultaneous detection sildenafil and its analogues was developed by using just one monoclonal antibody. This method can realize qualitative and quantitative analysis of sildenafil and its analogues with a detection range (IC20-IC80) of 0.12-28.05 ng mL-1, the IC50 was 0.31-8.45 ng mL-1 and the cut-off value was 5.0-80.0 ng mL-1. Meanwhile, this method could also accomplish semi-quantitative analysis of vardenafil with a cut-off value of 0.625 ng mL-1. The detection can be finished in 6 min and showed no cross-reactivity with avanafil and tadalafil. The recoveries of spiked samples ranged from 70.5 to 118.9% with a coefficient variation less than 15%, which confirmed the assay was sensitive, accurate and adapted to the rapid determination of sildenafil and its analogues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies / Qualitative_research Idioma: En Revista: Anal Chim Acta Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies / Qualitative_research Idioma: En Revista: Anal Chim Acta Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos