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Identification of a novel germ cell marker MnTdrd from the oriental river prawn Macrobrachium nipponense.
Dong, Yao-Ting; Feng, Hai-Yang; Tian, Xiao-Qing; Wang, Qi-Liang; Zhang, Shu-Fang; Ma, Ke-Yi; Qiu, Gao-Feng.
Afiliación
  • Dong YT; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
  • Feng HY; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
  • Tian XQ; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China.
  • Wang QL; College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong New Area, Shanghai, 201306, China.
  • Zhang SF; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
  • Ma KY; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
  • Qiu GF; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China.
Dev Genes Evol ; 231(1-2): 11-19, 2021 03.
Article en En | MEDLINE | ID: mdl-33244643
Germ cell-specific genes play an important role in establishing the reproductive system in sexual organisms and have been used as valuable markers for studying gametogenesis and sex differentiation. Previously, we isolated a vasa transcript as a germ cell marker to trace the origin and migration of germ cells in the oriental river prawn Macrobrachium nipponense. Here, we identified a new germ cell-specific marker MnTdrd RNA and assessed its temporal and spatial expression during oogenesis and embryogenesis. MnTdrd transcripts were expressed in high abundance in unfertilized eggs and embryos at cleavage stage and then dropped significantly during late embryogenesis, suggesting that MnTdrd mRNA is maternally inherited. In situ hybridization of ovarian tissue showed that MnTdrd mRNA was initially present in the cytoplasm of previtellogenic oocyte and localized to the perinuclear region as the accumulation of yolk in vitellogenic oocyte. Whole-mount in situ hybridization of embryos showed that MnTdrd-positive signals were only localized in one blastomere until 16-cell stage. In the blastula, there were approximately 16 MnTdrd-positive blastomeres. During embryonized-zoea stage, the MnTdrd-positive cells aggregated as a cluster and migrated to the genital rudiment which would develop into primordial germ cells (PGCs). The localized expression pattern of MnTdrd transcripts resembled that of the previously identified germ cell marker vasa, supporting the preformation mode of germ cell specification. Therefore, we concluded that MnTdrd, together with vasa, is a component of the germ plasm and might have critical roles in germ cell formation and differentiation in the prawn. Thus, MnTdrd can be used as a novel germ cell marker to trace the origin and migration of germ cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linaje de la Célula / Palaemonidae / Dominio Tudor / Células Germinativas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Dev Genes Evol Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linaje de la Célula / Palaemonidae / Dominio Tudor / Células Germinativas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Dev Genes Evol Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania