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Cardiac effect induced by Crotalus durissus cascavella venom: Morphofunctional evidence and mechanism of action.
Simões, Letícia O; Alves, Quiara L; Camargo, Samuel B; Araújo, Fênix A; Hora, Viviane R S; Jesus, Rafael L C; Barreto, Breno C; Macambira, Simone G; Soares, Milena B P; Meira, Cássio S; Aguiar, Márcio C; Couto, Ricardo D; Lomonte, Bruno; Menezes-Filho, José Evaldo; Cruz, Jader S; Vannier-Santos, Marcos A; Casais-E-Silva, Luciana L; Silva, Darizy F.
Afiliación
  • Simões LO; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Alves QL; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Camargo SB; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Araújo FA; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Hora VRS; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Jesus RLC; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Barreto BC; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil.
  • Macambira SG; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil; Department of Biochemistry and Biophysics, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Soares MBP; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil.
  • Meira CS; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil.
  • Aguiar MC; Department of Biomorphology, Institute of Health Sciences, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
  • Couto RD; Department of Clinical and Toxicological Analysis, Federal University of Bahia, Salvador, BA, 41170290, Brazil.
  • Lomonte B; Clodomiro Picado Institute, Faculty of Microbiology, University of Costa Rica, San José, 11501, Costa Rica.
  • Menezes-Filho JE; Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, MG, 30161970, Brazil.
  • Cruz JS; Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, MG, 30161970, Brazil.
  • Vannier-Santos MA; Oswaldo Cruz Institute, Oswaldo Cruz Foundation - IOC-Fiocruz, Rio de Janeiro, Brazil.
  • Casais-E-Silva LL; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil. Electronic address: luciana.casais@ufba.br.
  • Silva DF; Department of Bioregulation, Federal University of Bahia, Salvador, BA, 40110-902, Brazil.
Toxicol Lett ; 337: 121-133, 2021 Feb 01.
Article en En | MEDLINE | ID: mdl-33238178
Envenoming, resulting from snake bites, is a global public health problem. The present study was undertaken to investigate the influence of Crotalus durissus cascavella (Cdcas) venom on cardiac activity and the mechanisms of action underlying its effect. To investigate the inotropic and chronotropic effects induced by Cdcas, studies were performed on the left and right atria. A series of tests were conducted to investigate whether the negative inotropic effect, induced by Cdcas, was related to cardiac damage. Cdcas venom (0.1-30 µg/mL) elicited a significant negative inotropic effect. The addition of Cdcas crude venom (7.5, 15 and 30 µg/mL) did not induce significant alterations in cell proliferation, nor in the enzymatic activity of total-CK and CKMB. Ultrastructural evaluation demonstrated that cardiac cells from isoproterenol and Cdcas groups revealed discreet swelling and displaced intermyofibrillar mitochondria with disorganization of the cristae. No change was observed in cardiac electrical activity in perfused isolated rat hearts with Cdcas. In addition, Cdcas reduced contractility in isolated cardiomyocytes from the rat left ventricle. The negative inotropic effect of Cdcas was reduced by l-NAME (100 µM), PTIO (100 µM), ODQ (10 µM) and KT5823 (1 µM), suggesting the participation of NO/cGMP/PKG pathway due to Cdcas. In non-anesthetized rats, Cdcas induced hypotension followed by bradycardia, the latter was also observed by ECG (anesthetized animals). Our results suggest that the negative inotropic effect induced by Cdcas venom is unrelated to cardiac toxicity, at least, at the concentrations tested; and occurs through of NO/cGMP/PKG pathway, likely leading to hypotension and bradycardia when administered in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Crotalus / Venenos de Crotálidos / Corazón Límite: Animals Idioma: En Revista: Toxicol Lett Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Crotalus / Venenos de Crotálidos / Corazón Límite: Animals Idioma: En Revista: Toxicol Lett Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos