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Evaluating of HPV-DNA ISH as an adjunct to p16 testing in oropharyngeal cancer.
Chi, Jeffrey; Preeshagul, Isabel R; Sheikh-Fayyaz, Silvat; Teckie, Sewit; Kohn, Nina; Ziemba, Yonah; Laser, Alice; Frank, Douglas; Ghaly, Maged; Kamdar, Dev; Kraus, Dennis; Paul, Doru; Seetharamu, Nagashree.
Afiliación
  • Chi J; Department of Medical Oncology/Hematology, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Preeshagul IR; Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
  • Sheikh-Fayyaz S; Department of Pathology, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Teckie S; Department of Radiation Medicine, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Kohn N; Department of Biostatistics, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11030, USA.
  • Ziemba Y; Department of Pathology, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Laser A; Department of Pathology, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Frank D; Department of Otolaryngology - Head & Neck Surgery, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Ghaly M; Department of Pathology, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Kamdar D; Department of Otolaryngology - Head & Neck Surgery, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Kraus D; Department of Otolaryngology - Head & Neck Surgery, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Paul D; Department of Medical Oncology/Hematology, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
  • Seetharamu N; Department of Medical Oncology/Hematology, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, New York, NY 11042, USA.
Future Sci OA ; 6(9): FSO606, 2020 Aug 03.
Article en En | MEDLINE | ID: mdl-33235805
AIM: Current guidelines recommend p16 immunohistochemistry (IHC) for testing human papillomavirus (HPV) in oropharyngeal carcinoma (OPSCC). We evaluated the value of adding DNA in situ hybridization (ISH) to p16 IHC. METHODS: Fifty patients with OPSCC were analyzed. Concordance between HPV-DNA ISH and p16 IHC was measured by Gwet's agreement coefficient. RESULTS: p16 IHC was positive in 35/48 (72.9%), negative in 8/48 (16.7%) patients. Wide spectrum DNA-ISH was positive in 9/23 (39%) and negative in 14/23 (60.9%) patients. High-risk 16/18 (HR) HPV DNA-ISH was positive in 11/23 (47.8%) and negative in 12 (52.2%) patients. The agreement between HPV DNA-ISH and p16 IHC is fair (Gwet's AC1 = 0.318). CONCLUSION: The agreement between p16 IHC and HPV-DNA ISH was fair. However, ISH sensitivity was low. Our findings add to the current data that p16 IHC testing is reliable and may be enough as a stand-alone test for HPV detection in OPSCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Future Sci OA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Future Sci OA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido