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Genomic Characterization of Interspecific Hybrids and an Admixture Population Derived from Panicum amarum × P. virgatum.
Heffelfinger, Christopher; Deresienski, Adam P; Nelson, Kimberly A; Moreno, Maria A; Hague, Joel P; Dellaporta, Stephen L; Kausch, Albert P.
Afiliación
  • Heffelfinger C; Dep. of Molecular, Cellular, and Developmental Biology, Yale Univ., New Haven, CT, 06511.
  • Deresienski AP; Dep. of Cell and Molecular Biology, Univ. of Rhode Island, Kingston, RI, 02892.
  • Nelson KA; Dep. of Cell and Molecular Biology, Univ. of Rhode Island, Kingston, RI, 02892.
  • Moreno MA; Dep. of Molecular, Cellular, and Developmental Biology, Yale Univ., New Haven, CT, 06511.
  • Hague JP; Dep. of Cell and Molecular Biology, Univ. of Rhode Island, Kingston, RI, 02892.
  • Dellaporta SL; Dep. of Molecular, Cellular, and Developmental Biology, Yale Univ., New Haven, CT, 06511.
  • Kausch AP; Dep. of Cell and Molecular Biology, Univ. of Rhode Island, Kingston, RI, 02892.
Plant Genome ; 8(2): eplantgenome2015.01.0001, 2015 Jul.
Article en En | MEDLINE | ID: mdl-33228322
Switchgrass (Panicum virgatum L.) and its relatives are regarded as top bioenergy crop candidates; however, one critical barrier is the introduction of useful genetic diversity and the development of new cultivars and hybrids. Combining genomes from related cultivars and species provides an opportunity to introduce new traits. In switchgrass, a breeding advantage would be achieved by combining the genomes of intervarietal ecotypes or interspecific hybrids. The recovery of wide crosses, however, is often tedious and may involve complicated embryo rescue and numerous backcrosses. Here, we demonstrate a straightforward approach to wide crosses involving the use of a selectable transgene for recovery of interspecific [P. virgatum cv. Alamo × Panicum amarum Ell. var amarulum or Atlantic Coastal Panicgrass (ACP)] F1 hybrids followed by backcrossing to generate a nontransgenic admixture population. A nontransgenic herbicide-sensitive (HbS) admixture population of 83 F1 BC1 progeny was analyzed by genotyping-by-sequencing (GBS) to characterize local ancestry, parental contribution, and patterns of recombination. These results demonstrate a widely applicable breeding strategy that makes use of transgenic selectable resistance to identify and recover true hybrids.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Genome Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Genome Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos