Oriented Immobilization of Protein Templates: A New Trend in Surface Imprinting.
ACS Sens
; 5(12): 3710-3720, 2020 12 24.
Article
en En
| MEDLINE
| ID: mdl-33225686
In this Review, we have summarized recent trends in protein template imprinting. We emphasized a new trend in surface imprinting, namely, oriented protein immobilization. Site-directed proteins were assembled through specially selected functionalities. These efforts resulted in a preferably oriented homogeneous protein construct with decreased protein conformation changes during imprinting. Moreover, the maximum functionality for protein recognition was utilized. Various strategies were exploited for oriented protein immobilization, including covalent immobilization through a boronic acid group, metal coordinating center, and aptamer-based immobilization. Moreover, we have discussed the involvement of semicovalent as well as covalent imprinting. Interestingly, these approaches provided additional recognition sites in the molecular cavities imprinted. Therefore, these molecular cavities were highly selective, and the binding kinetics was improved.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Impresión Molecular
Idioma:
En
Revista:
ACS Sens
Año:
2020
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Estados Unidos