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Monodisperse microsphere-based immobilized metal affinity chromatography approach for preparing Antarctic krill phospholipids followed by HILIC-MS analysis.
Shen, Qing; Wu, Huanming; Wang, Honghai; Zhao, Qiaoling; Xue, Jing; Ma, Jianfeng; Wang, Haixing.
Afiliación
  • Shen Q; Zhejiang Province Key Laboratory of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China; Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institut
  • Wu H; Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, Ningbo University, Ningbo, Zhejiang 315211, China.
  • Wang H; Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.
  • Zhao Q; Zhoushan Institute for Food & Drug Control, Zhoushan 316000, China.
  • Xue J; Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address: xuejing@zjgsu.edu.cn.
  • Ma J; Zhejiang Province Key Laboratory of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
  • Wang H; Zhejiang Province Key Laboratory of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address: wanghaixing@wmu.edu.cn.
Food Chem ; 344: 128585, 2021 May 15.
Article en En | MEDLINE | ID: mdl-33223290
Phospholipids enriched krill is a functional food beneficial in cardiovascular diseases. Herein, monodisperse microsphere-based immobilized metal affinity chromatographic material (MM-IMAC) was synthesized with Ti4+ incorporated to enrich phospholipids from krill by coordination with phosphate group. The extract was profiled by hydrophilic interaction chromatography-mass spectrometry (HILIC-MS) with 154 phospholipid molecular species detected. The parameters were loading solvent n-hexane/isopropanol (2:8, v/v), flow rate 0.8 mL·min-1, and eluting volume 1 mL. Besides, eicosapentaenoic and docosahexaenoic acids structured phospholipids were located, such as phosphatidylcholine (PC) 20:5/22:6, phosphatidylinositol (PI) 18:0/20:5, etc. Finally, this method was validated in linearity (R2 ≥ 0.9953), sensitivity (LOD ≤ 0.53 µg·mL-1 and LOQ ≤ 1.66 µg·mL-1), precision (RSDintraday ≤ 4.86% and RSDinterday ≤ 6.25%), and recovery (58-83%). It indicated that the MM-Ti4+-IMAC-HILIC-MS was reliable and efficient in specific study of phospholipids in food matrix.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolípidos / Cromatografía Líquida de Alta Presión / Euphausiacea / Microesferas Límite: Animals Idioma: En Revista: Food Chem Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolípidos / Cromatografía Líquida de Alta Presión / Euphausiacea / Microesferas Límite: Animals Idioma: En Revista: Food Chem Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido