Quantitative determination of urinary metabolites of geraniol by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
Anal Methods
; 12(47): 5718-5728, 2020 12 21.
Article
en En
| MEDLINE
| ID: mdl-33220670
Geraniol is a fragrance which occurs in natural terpene oil or is chemically synthesized on a large scale. It is used in a wide variety of consumer products such as perfumes, deodorants, household products and cosmetics. Hence, not only industry workers in the production of geraniol, but also consumers can come into contact with the substance. Human biomonitoring (HBM), i.e. the analytical determination of substances and their metabolites in human biological material, is a key element in the analysis and assessment of the distribution and intensity of occupational and environmental exposure of humans. Therefore, a procedure for the quantitative determination of the urinary metabolites Hildebrandt acid, geranic acid, 3-hydroxycitronellic acid and 8-carboxygeraniol as potential biomarkers of geraniol exposure was developed and validated. The method is based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) after enzymatic hydrolysis and liquid-liquid extraction (LLE) of the target analytes. The limit of quantification (LOQ) is 1.5 µg L-1 for 8-carboxygeraniol, 2.7 µg L-1 each for Hildebrandt acid and geranic acid, and 1.8 µg L-1 for 3-hydroxycitronellic acid. The method was applied to urine samples of 41 persons without occupational exposure to geraniol. Hildebrandt acid and geranic acid were detected in all samples, 8-carboxygeraniol in 83% and 3-hydroxycitronellic acid in 81% of the samples. Hildebrandt acid (median: 313 µg L-1, range: 37-1966 µg L-1) was the most abundant metabolite, followed by geranic acid (93 µg L-1; 9-477 µg L-1), 3-hydroxycitronellic acid (18 µg L-1;
Asunto(s)
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas en Tándem
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Methods
Año:
2020
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas en Tándem
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Methods
Año:
2020
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Reino Unido