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Maize transcription factor ZmBES1/BZR1-5 positively regulates kernel size.
Sun, Fuai; Ding, Lei; Feng, Wenqi; Cao, Yang; Lu, Fengzhong; Yang, Qingqing; Li, Wanchen; Lu, Yanli; Shabek, Nitzan; Fu, Fengling; Yu, Haoqiang.
Afiliación
  • Sun F; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Ding L; Department of Plant Biology, University of California, Davis, CA, USA.
  • Feng W; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Cao Y; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Lu F; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Yang Q; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Li W; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Lu Y; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Shabek N; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • Fu F; Department of Plant Biology, University of California, Davis, CA, USA.
  • Yu H; Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture; Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
J Exp Bot ; 72(5): 1714-1726, 2021 02 27.
Article en En | MEDLINE | ID: mdl-33206180
The BES1/BZR1 transcription factors regulate the expression of genes responsive to brassinosteroids and play pivotal roles in plant development, but their role in regulating kernel development in maize remains unclear. In this study, we found that ZmBES1/BZR1-5 positively regulates kernel size. Association analysis of candidate genes in 513 diverse maize inbred lines indicated that three SNPs related to ZmBES1/BZR1-5 were significantly associated with kernel width and whilst four SNPs were related to 100-kernel weight. Overexpression of ZmBES1/BZR1-5 in Arabidopsis and rice both significantly increased seed size and weight, and smaller kernels were produced in maize Mu transposon insertion and EMS mutants. The ZmBES1/BZR1-5 protein locates in the nucleus, contains bHLH and BAM domains, and shows no transcriptional activity as a monomer but forms a homodimer through the BAM domain. ChIP-sequencing analysis, and yeast one-hybrid and dual-luciferase assays demonstrated that the protein binds to the promoters of AP2/EREBP genes (Zm00001d010676 and Zm00001d032077) and inhibits their transcription. cDNA library screening showed that ZmBES1/BZR1-5 interacts with casein kinase II subunit ß4 (ZmCKIIß4) and ferredoxin 2 (ZmFdx2) in vitro and in vivo, respectively. Taken together, our study suggests that ZmBES1/BZR1-5 positively regulates kernel size, and provides new insights into understanding the mechanisms of kernel development in maize.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Semillas / Factores de Transcripción / Zea mays Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Semillas / Factores de Transcripción / Zea mays Idioma: En Revista: J Exp Bot Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido