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Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test.
Heggestad, Jacob T; Kinnamon, David S; Olson, Lyra B; Liu, Jason; Kelly, Garrett; Wall, Simone A; Fontes, Cassio M; Joh, Daniel Y; Hucknall, Angus M; Pieper, Carl; Naqvi, Ibtehaj A; Chen, Lingye; Que, Loretta G; Oguin, Thomas; Nair, Smita K; Sullenger, Bruce A; Woods, Christopher W; Sempowski, Gregory D; Kraft, Bryan D; Chilkoti, Ashutosh.
Afiliación
  • Heggestad JT; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Kinnamon DS; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Olson LB; Duke Medical Scientist Training Program, Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC 27710 USA.
  • Liu J; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Kelly G; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Wall SA; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Fontes CM; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Joh DY; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Hucknall AM; Division of Plastic, Maxillofacial, and Oral Surgery, Department of Surgery, Duke University Medical Center, Durham NC 27710 USA.
  • Pieper C; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Naqvi IA; Departments of Biostatistics and Bioinformatics, Duke University Medical Center, Durham, NC 27708 USA.
  • Chen L; Department of Surgery, Duke University School of Medicine, Durham, NC 27710 USA.
  • Que LG; Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, Duke University Medical Center, Durham, NC 27710 USA.
  • Oguin T; Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, Duke University Medical Center, Durham, NC 27710 USA.
  • Nair SK; Department of Medicine and Duke Human Vaccine Institute, School of Medicine, Duke University, Durham, NC 27710 USA.
  • Sullenger BA; Department of Surgery, Duke University School of Medicine, Durham, NC 27710 USA.
  • Woods CW; Department of Neurosurgery and Pathology, Duke University School of Medicine, Duke University, Durham, NC 27710 USA.
  • Sempowski GD; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708 USA.
  • Kraft BD; Department of Surgery, Duke University School of Medicine, Durham, NC 27710 USA.
  • Chilkoti A; Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC 27710 USA.
medRxiv ; 2020 Nov 07.
Article en En | MEDLINE | ID: mdl-33173900
Highly sensitive, specific, and point-of-care (POC) serological assays are an essential tool to manage the COVID-19 pandemic. Here, we report on a microfluidic, multiplexed POC test that can profile the antibody response against multiple SARS-CoV-2 antigens - Spike S1 (S1), Nucleocapsid (N), and the receptor binding domain (RBD) - simultaneously from a 60 microliter drop of blood, plasma, or serum. We assessed the levels of anti-SARS-CoV-2 antibodies in plasma samples from 19 individuals (at multiple time points) with COVID-19 that required admission to the intensive care unit and from 10 healthy individuals. This POC assay shows good concordance with a live virus microneutralization assay, achieved high sensitivity (100%) and specificity (100%), and successfully tracked the longitudinal evolution of the antibody response in infected individuals. We also demonstrated that we can detect a chemokine, IP-10, on the same chip, which may provide prognostic insight into patient outcomes. Because our test requires minimal user intervention and is read by a handheld detector, it can be globally deployed in the fight against COVID-19 by democratizing access to laboratory quality tests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: MedRxiv Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: MedRxiv Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos