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Exonuclease 5 is dispensable for meiotic progression and male fertility in mouse.
Zaman, Qumar; Khan, Ranjha; Hameed, Uzma; Shah, Wasim; Waqas, Ahmed; Zubair, Muhammad; Dil, Sobia; Zafar, Tuba; Ali, Asim; Unar, Ahsanullah; Shi, Qinghua.
Afiliación
  • Zaman Q; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.
  • Khan R; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.
  • Hameed U; Institute of Industrial Biotechnology, Government College University Lahore, Pakistan.
  • Shah W; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.
  • Waqas A; Department of Zoology, Division of Science and Technology, University of Education Lahore, Multan Campus, Multan, Pakistan.
  • Zubair M; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.
  • Dil S; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.
  • Zafar T; Institute of Industrial Biotechnology, Government College University Lahore, Pakistan.
  • Ali A; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.
  • Unar A; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China.
  • Shi Q; The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at Microscale, School of Basic Medical Sciences, University of Science and Technology of China, Hefei 230027, China. Electronic address: qshi@ustc.edu.cn.
Gene ; 769: 145254, 2021 Feb 15.
Article en En | MEDLINE | ID: mdl-33164760
Exonuclease 5 (Exo5) belongs to a class of bi-directional, ssDNA-specific exonucleases that mainly involved in the DNA repair pathways. Exo5 has been reported to be crucial for DNA- DNA mismatch repair (MMR) in several human cell lines. However, its in vivo function in mammals still needs to be explored. Thus, to study the in vivo role of Exo5 in spermatogenesis, Exo5 knockout mice were generated using CRISPR/Cas9 technology. Unexpectedly, we found that the knockout mice are fertile despite a slight decrease in sperm count. Furthermore, Exo5-/- mice showed no detectable developmental anomalies, exhibited no remarkable differences in the epididymal histology and testis/body weight ratio. Moreover, cytological investigations on meiocytes revealed non-significant differences in chromosomal synapsis, recombination, and meiotic progression of prophase I, further demonstrating that Exo5 has no essential role in spermatogenesis in mice under normal breeding conditions. Collectively, these data indicate that Exo5 is dispensable for meiotic progression and fertility in mice.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exodesoxirribonucleasa V / Fertilidad / Meiosis Límite: Animals Idioma: En Revista: Gene Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exodesoxirribonucleasa V / Fertilidad / Meiosis Límite: Animals Idioma: En Revista: Gene Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos