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Rapid Quantification of Gut Microbial Short-Chain Fatty Acids by pDART-MS.
Weng, Cheng-Yu; Kuo, Ting-Hao; Chai, Laura Min Xuan; Zou, Hsin-Bai; Feng, Tzu-Hsuan; Huang, Yun-Ju; Tsai, Jemmy C; Wu, Ping-Hsun; Chiu, Yi-Wen; Lan, Ethan I; Sheen, Lee-Yan; Hsu, Cheng-Chih.
Afiliación
  • Weng CY; Department of Chemistry, National Taiwan University, Taipei 106216, Taiwan.
  • Kuo TH; Department of Chemistry, National Taiwan University, Taipei 106216, Taiwan.
  • Chai LMX; Department of Chemistry, National Taiwan University, Taipei 106216, Taiwan.
  • Zou HB; Department of Chemistry, National Taiwan University, Taipei 106216, Taiwan.
  • Feng TH; Department of Chemistry, National Taiwan University, Taipei 106216, Taiwan.
  • Huang YJ; Institute of Food Science and Technology, National Taiwan University, Taipei 106216, Taiwan.
  • Tsai JC; Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 300093, Taiwan.
  • Wu PH; Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807378, Taiwan.
  • Chiu YW; Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807378, Taiwan.
  • Lan EI; Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 300093, Taiwan.
  • Sheen LY; Institute of Food Science and Technology, National Taiwan University, Taipei 106216, Taiwan.
  • Hsu CC; Department of Chemistry, National Taiwan University, Taipei 106216, Taiwan.
Anal Chem ; 92(22): 14892-14897, 2020 11 17.
Article en En | MEDLINE | ID: mdl-33151059
Short-chain fatty acids (SCFAs) are small molecules ubiquitous in nature. In mammalian guts, SCFAs are mostly produced by anaerobic intestinal microbiota through the fermentation of dietary fiber. Levels of microbe-derived SCFAs are closely relevant to human health status and indicative to gut microbiota dysbiosis. However, the quantification of SCFA using conventional chromatographic approaches is often time consuming, thus limiting high-throughput screening tests. Herein, we established a novel method to quantify SCFAs by coupling amidation derivatization of SCFAs with paper-loaded direct analysis in real time mass spectrometry (pDART-MS). Remarkably, SCFAs of a biological sample were quantitatively determined within a minute using the pDART-MS platform, which showed a limit of detection at the µM level. This platform was applied to quantify SCFAs in various biological samples, including feces from stressed rats, sera of patients with kidney disease, and fermentation products of metabolically engineered cyanobacteria. Significant differences in SCFA levels between different groups of biological practices were promptly revealed and evaluated. As there is a burgeoning demand for the analysis of SCFAs due to an increasing academic interest of gut microbiota and its metabolism, this newly developed platform will be of great potential in biological and clinical sciences as well as in industrial quality control.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Ácidos Grasos Volátiles / Microbioma Gastrointestinal Aspecto: Patient_preference Límite: Humans Idioma: En Revista: Anal Chem Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Ácidos Grasos Volátiles / Microbioma Gastrointestinal Aspecto: Patient_preference Límite: Humans Idioma: En Revista: Anal Chem Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos