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Depth-resolved Mueller matrix polarimetry microscopy of the rat cornea.
Du Le, V N; Saytashev, Ilyas; Saha, Sudipta; Lopez, Pedro F; Laughrey, Megan; Ramella-Roman, Jessica C.
Afiliación
  • Du Le VN; Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA.
  • Saytashev I; vidule@fiu.edu.
  • Saha S; Department of Ophthalmology, Herbert Wertheim College of Medicine, Florida International University, 11200 SW 8 Street, Miami, FL 33199, USA.
  • Lopez PF; Department of Biomedical Engineering, College of Engineering and Computing, Florida International University, 10555 West Flagler Street, Miami, FL 33174, USA.
  • Laughrey M; Department of Ophthalmology, Herbert Wertheim College of Medicine, Florida International University, 11200 SW 8 Street, Miami, FL 33199, USA.
  • Ramella-Roman JC; Department of Ophthalmology, Herbert Wertheim College of Medicine, Florida International University, 11200 SW 8 Street, Miami, FL 33199, USA.
Biomed Opt Express ; 11(10): 5982-5994, 2020 Oct 01.
Article en En | MEDLINE | ID: mdl-33150000
Mueller matrix polarimetry (MMP) is a promising linear imaging modality that can enable visualization and measurement of the polarization properties of the cornea. Although the distribution of corneal birefringence has been reported, depth resolved MMP imaging of the cornea has not been archived and remains challenging. In this work, we perform depth-resolved imaging of the cornea using an improved system that combines Mueller matrix reflectance and transmission microscopy together with nonlinear microscopy utilizing second harmonic generation (SHG) and two photon excitation fluorescence (TPEF). We show that TPEF can reveal corneal epithelial cellular network while SHG can highlight the presence of corneal stromal lamellae. We then demonstrate that, in confocal reflectance measurement, as depth increases from 0 to 80 µm both corneal depolarization and retardation increase. Furthermore, it is shown that the spatial distribution of corneal depolarization and retardation displays similar complexity in both reflectance (confocal and non-confocal) and transmission measurement, likely due to the strong degree of heterogeneity in the stromal lamellae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos