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Morphological and anatomical characteristics of exserted stigma sterility and the location and function of SlLst (Solanum lycopersicum Long styles) gene in tomato.
Cheng, Mo-Zhen; Gong, Chao; Zhang, Bo; Qu, Wei; Qi, Hao-Nan; Chen, Xiu-Ling; Wang, Xing-Yuan; Zhang, Yao; Liu, Jia-Yin; Ding, Xiao-Dong; Qiu, You-Wen; Wang, Ao-Xue.
Afiliación
  • Cheng MZ; College of Life Sciences, Northeast Agricultural University, Harbin, China.
  • Gong C; College of Life Sciences, Northeast Agricultural University, Harbin, China.
  • Zhang B; Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
  • Qu W; College of Life Sciences, Northeast Agricultural University, Harbin, China.
  • Qi HN; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China.
  • Chen XL; College of Life Sciences, Northeast Agricultural University, Harbin, China.
  • Wang XY; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China.
  • Zhang Y; College of Life Sciences, Northeast Agricultural University, Harbin, China.
  • Liu JY; College of Life Sciences, Northeast Agricultural University, Harbin, China.
  • Ding XD; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China.
  • Qiu YW; College of Life Sciences, Northeast Agricultural University, Harbin, China.
  • Wang AX; College of Life Sciences, Northeast Agricultural University, Harbin, China.
Theor Appl Genet ; 134(2): 505-518, 2021 Feb.
Article en En | MEDLINE | ID: mdl-33140169
KEY MESSAGE: Anatomical changes in and hormone roles of the exserted stigma were investigated, and localization and functional analysis of SlLst for the exserted stigma were performed using SLAF-BSA-seq, parental resequencing and overexpression of SlLst in tomato. Tomato accession T431 produces stigmas under relatively high temperatures (> 27 °C, the average temperature in Harbin, China, in June-August), so pollen can rarely reach the stigma properly. This allows the percentage of male sterility exceed 95%, making the use of this accession practical for hybrid seed production. To investigate the mechanism underlying the exserted stigma male sterility, the morphological changes of, anatomical changes of, and comparative endogenous hormone (IAA, ABA, GA3, ZT, SA) changes in flowers during flower development of tomato accessions DL5 and T431 were measured. The location and function of genes controlling exserted stigma sterility were analyzed using super SLAF-BSA-seq, parental resequencing, comparative genomics and the overexpression of SlLst in tomato. The results showed that an increase in cell number mainly caused stigma exsertion. IAA played a major role, while ABA had an opposite effect on stigma exertion. Moreover, 26 candidate genes related to the exserted stigma were found, located on chromosome 12. The Solyc12g027610.1 (SlLst) gene was identified as the key candidate gene by functional analysis. A subcellular localization assay revealed that SlLst is targeted to the nucleus and cell membrane. Phenotypic analysis of SlLst-overexpressing tomato showed that SlLst plays a crucial role during stigma exsertion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Semillas / Solanum lycopersicum / Regulación de la Expresión Génica de las Plantas / Flores / Sitios de Carácter Cuantitativo / Infertilidad Vegetal Tipo de estudio: Prognostic_studies Idioma: En Revista: Theor Appl Genet Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Semillas / Solanum lycopersicum / Regulación de la Expresión Génica de las Plantas / Flores / Sitios de Carácter Cuantitativo / Infertilidad Vegetal Tipo de estudio: Prognostic_studies Idioma: En Revista: Theor Appl Genet Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania