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A dynamic mechanical method to assess bulk viscoelastic behavior of the dentin extracellular matrix.
Alania, Yvette; Reis, Mariana Cavalcante Dos; Nam, Joo-Won; Phansalkar, Rasika S; McAlpine, James; Chen, Shao-Nong; Pauli, Guido F; Bedran-Russo, Ana K.
Afiliación
  • Alania Y; Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA; Department of General Dental Sciences, School of Dentistry, Marquette University, Milwaukee, WI 53233, USA.
  • Reis MCD; Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA; Department of General Dental Sciences, School of Dentistry, Marquette University, Milwaukee, WI 53233, USA.
  • Nam JW; Department of Pharmaceutical Sciences and Pharmacognosy Institute, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Phansalkar RS; Department of Pharmaceutical Sciences and Pharmacognosy Institute, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • McAlpine J; Department of Pharmaceutical Sciences and Pharmacognosy Institute, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Chen SN; Department of Pharmaceutical Sciences and Pharmacognosy Institute, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Pauli GF; Department of Pharmaceutical Sciences and Pharmacognosy Institute, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Bedran-Russo AK; Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA; Department of General Dental Sciences, School of Dentistry, Marquette University, Milwaukee, WI 53233, USA. Electronic address: ana.bedran-russo@marquette.edu.
Dent Mater ; 36(12): 1536-1543, 2020 12.
Article en En | MEDLINE | ID: mdl-33129510
OBJECTIVES: To develop a protocol for assessment of the bulk viscoelastic behavior of dentin extracellular matrix (ECM), and to assess relationships between induced collagen cross-linking and viscoelasticity of the dentin ECM. METHODS: Dentin ECM was treated with agents to induce exogenous collagen cross-linking: proanthocyanidins (PACs) from Vitis vinifera - VVe, PACs from Pinus massoniana - PMe, glutaraldehyde - (GA), or kept untreated (control). A dynamic mechanical strain sweep method was carried out in a 3-point bending submersion clamp at treatment; after protein destabilization with 4 M urea and after 7-day, 6-month, and 12-month incubation in simulated body fluid. Tan δ, storage (E'), loss (E"), and complex moduli (E*) were calculated and data were statistically analyzed using two-way ANOVA and post-hoc tests (α = 0.05). Chemical analysis of dentin ECM before and after protein destabilization was assessed with ATR-FTIR spectroscopy. RESULTS: Significant interactions between study factors (treatment vs. time points, p < 0.001) were found for all viscoelastic parameters. Despite a significant decrease in all moduli after destabilization, PAC-treated dentin remained statistically higher than control (p < 0.001), indicating permanent mechanical enhancement after biomodification. Covalently crosslinked, GA-treated dentin was unaffected by destabilization (p = 0.873) and showed the lowest damping capacity (tan δ) at all time points (p < 0.001). After 12 months, the damping capacity of PMe and VVe groups decreased significantly. Changes in all amide IR resonances revealed a partial chemical reversal of PAC-mediated biomodification. SIGNIFICANCE: Viscoelastic measurements and IR spectroscopy aid in elucidating the role of inter-molecular collagen cross-linking in the mechanical behavior of dentin ECM.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proantocianidinas / Extracto de Semillas de Uva Tipo de estudio: Guideline Idioma: En Revista: Dent Mater Asunto de la revista: ODONTOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proantocianidinas / Extracto de Semillas de Uva Tipo de estudio: Guideline Idioma: En Revista: Dent Mater Asunto de la revista: ODONTOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido