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One-step rapid quantification of SARS-CoV-2 virus particles via low-cost nanoplasmonic sensors in generic microplate reader and point-of-care device.
Huang, Liping; Ding, Longfei; Zhou, Jun; Chen, Shuiliang; Chen, Fang; Zhao, Chen; Xu, Jianqing; Hu, Wenjun; Ji, Jiansong; Xu, Hao; Liu, Gang L.
Afiliación
  • Huang L; National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luo Yu Road, Wuhan, 430074, PR China; Liangzhun (Shanghai) Industrial Co. Ltd, Shanghai, China. Electronic address: lphuang@aliyun.com.
  • Ding L; Shanghai Public Health Clinical Center, Fudan University, China.
  • Zhou J; Wuhan Xinxin Semiconductor Manufacturing Co. Ltd, Wuhan, China.
  • Chen S; Taiwan Semiconductor Manufacturing Co., Shanghai, China.
  • Chen F; Taiwan Semiconductor Manufacturing Co., Shanghai, China.
  • Zhao C; Shanghai Public Health Clinical Center, Fudan University, China.
  • Xu J; Shanghai Public Health Clinical Center, Fudan University, China.
  • Hu W; National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luo Yu Road, Wuhan, 430074, PR China.
  • Ji J; Lishui Central Hospital, Zhejiang University, Zhejiang, China.
  • Xu H; Liangzhun (Shanghai) Industrial Co. Ltd, Shanghai, China. Electronic address: billxu@kaizzi.com.
  • Liu GL; National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luo Yu Road, Wuhan, 430074, PR China. Electronic address: loganliu@hust.edu.cn.
Biosens Bioelectron ; 171: 112685, 2021 Jan 01.
Article en En | MEDLINE | ID: mdl-33113383
The spread of SARS-CoV-2 virus in the ongoing global pandemic has led to infections of millions of people and losses of many lives. The rapid, accurate and convenient SARS-CoV-2 virus detection is crucial for controlling and stopping the pandemic. Diagnosis of patients in the early stage infection are so far limited to viral nucleic acid or antigen detection in human nasopharyngeal swab or saliva samples. Here we developed a method for rapid and direct optical measurement of SARS-CoV-2 virus particles in one step nearly without any sample preparation using a spike protein specific nanoplasmonic resonance sensor. As low as 370 vp/mL were detected in one step within 15 min and the virus concentration can be quantified linearly in the range of 0 to 107 vp/mL. Measurements shown on both generic microplate reader and a handheld smartphone connected device suggest that our low-cost and rapid detection method may be adopted quickly under both regular clinical environment and resource-limited settings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Viral / Virión / Técnicas Biosensibles / Infecciones por Coronavirus / Técnicas de Laboratorio Clínico / Pruebas en el Punto de Atención / Betacoronavirus Tipo de estudio: Diagnostic_studies / Evaluation_studies / Health_economic_evaluation / Prognostic_studies Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Viral / Virión / Técnicas Biosensibles / Infecciones por Coronavirus / Técnicas de Laboratorio Clínico / Pruebas en el Punto de Atención / Betacoronavirus Tipo de estudio: Diagnostic_studies / Evaluation_studies / Health_economic_evaluation / Prognostic_studies Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido