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Bead-Based Extracellular Vesicle Analysis Using Flow Cytometry.
Yang, Katherine S; Lin, Hsing-Ying; Curley, Caleigh; Welch, Marisa W; Wolpin, Brian M; Lee, Hakho; Weissleder, Ralph; Im, Hyungsoon; Castro, Cesar M.
Afiliación
  • Yang KS; Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.
  • Lin HY; Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114, USA.
  • Curley C; Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.
  • Welch MW; Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.
  • Wolpin BM; Dana-Farber Cancer Institute, Boston, MA, 02115, USA.
  • Lee H; Dana-Farber Cancer Institute, Boston, MA, 02115, USA.
  • Weissleder R; Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.
  • Im H; Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114, USA.
  • Castro CM; Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.
Adv Biosyst ; 4(12): e2000203, 2020 12.
Article en En | MEDLINE | ID: mdl-33103361
Extracellular vesicles (EVs) represent promising circulating biomarkers for cancers, but their high-throughput analyses in clinical settings prove challenging due to lack of simple, fast, and robust EV assays. Here, a bead-based EV assay detected by flow cytometry is described, which integrates EV capture using microbeads with EV protein analyses by flow cytometry. The assay is fast (<4 h for 48 samples), robust, and compatible with conventional flow cytometry instruments for high-throughput EV analysis. With the method, a panel of pancreatic cancer biomarkers in EVs from plasma samples of pancreatic cancer patients is successfully analyzed. The assay is readily translatable to other biomarkers or cancer types and can be run with standard materials on conventional flow cytometers, making it highly flexible and adaptable to diverse research and clinical needs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citometría de Flujo / Vesículas Extracelulares Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Adv Biosyst Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citometría de Flujo / Vesículas Extracelulares Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Adv Biosyst Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania