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Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum.
El-Sayed, Ashraf; Enan, Gamal; Al-Mohammadi, Abdul-Raouf; H Moustafa, Ahmed; El-Gazzar, Nashwa.
Afiliación
  • El-Sayed A; Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
  • Enan G; Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
  • Al-Mohammadi AR; Chemistry Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
  • H Moustafa A; Department of Sciences, King Khalid Military Academy, Riyadh 11495, Saudi Arabia.
  • El-Gazzar N; Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
Molecules ; 25(20)2020 Oct 20.
Article en En | MEDLINE | ID: mdl-33092293
Penicillium chrysogenum has been reported as a potent taxol producer based on quantitative analysis by TLC and HPLC. The biosynthetic potency of taxol has been validated from PCR detection of rate-limiting genes of taxol synthesis such as taxadienesynthase and 10-de-acetylbaccatin III-O-acetyltransferase (DBAT), which catalyzes the immediate diterpenoid precursor of the taxol substance, as detected by PCR. Taxol production by P. chrysogenum was assessed by growing the fungus on different media. Potato dextrose broth (PDB) was shown to be the best medium for obtaining the higher amount of taxol (170 µg/L). A stepwise optimization of culture conditions necessary for production of higher amounts of taxol was investigated. The substance taxol was produced optimally after 18 d of incubation at 30 °C in PDB adjusted initially at pH 8.0 with shaking (120 rpm) (250 µg/L). The P. chrysogenum taxol was purified successfully by HPLC. Instrumental analyzes such as Fourier transform infrared spectroscopy (FTIR), ultraviolet (UV) spectroscopy, 1HNMR and 13C NMR approved the structural formula of taxol (C47H51NO14), as constructed by ChemDraw. The P. chrysogenum taxol showed promising anticancer activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicillium chrysogenum / Paclitaxel / Proliferación Celular Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicillium chrysogenum / Paclitaxel / Proliferación Celular Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza