Genotypic profile of benzimidazole resistance associated with SNP F167Y in the beta-tubulin gene of Necator americanus helminths obtained from Brazilian populations.

Furtado, Luis Fernando Viana; Dos Santos, Talita Rodrigues; de Oliveira, Valéria Nayara Gomes Mendes; Rabelo, Élida Mara Leite

Furtado, Luis Fernando Viana; Dos Santos, Talita Rodrigues; de Oliveira, Valéria Nayara Gomes Mendes; Rabelo, Élida Mara Leite.

Afiliación

Furtado LFV; Universidade do Estado de Minas Gerais, Avenida Juca Stockler, 1130, CEP 37900-106, Nossa Sra. das Graças, Passos, Minas Gerais, Brazil.
Dos Santos TR; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Avenida Presidente Antônio Carlos, 6627, Departamento de Parasitologia, L4 237, Laboratório de Parasitologia Molecular, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
de Oliveira VNGM; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Avenida Presidente Antônio Carlos, 6627, Departamento de Parasitologia, L4 237, Laboratório de Parasitologia Molecular, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil.
Rabelo ÉML; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Avenida Presidente Antônio Carlos, 6627, Departamento de Parasitologia, L4 237, Laboratório de Parasitologia Molecular, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil. Electronic address: rabelo@icb.ufmg.br.

Infect Genet Evol ; 86: 104594, 2020 12.

Article en En | MEDLINE | ID: mdl-33080381

RESUMEN

Necator americanus is a worm that parasites the small intestine of humans and is highly prevalent in regions with poor sanitary conditions. The main strategy to control this helminth is by mass benzimidazole administration, however, periodic use of these drugs can select strains of parasites resistant to treatment. Single nucleotide polymorphisms (SNPs) in the beta-tubulin isotype 1 gene located at codons 167, 198 and 200 have been associated with benzimidazole resistance in some nematodes. Previously, our group detected the presence of some of these SNPs in populations of soil-transmitted helminths collected in different locations in Brazil. Here, we evaluated the SNP at codon 167, which has recently been shown to be associated with failure of benzimidazoles to treat N. americanus. Our ARMS-PCR analyses were performed using 524 single N. americanus eggs from 48 patients' feces collected in six Brazilian states; however, we did not detect any mutated samples at codon 167. This study builds on previous work, helping us monitor the presence of resistance-related genotypes in Brazilian helminth populations. The data presented here can assist in the implementation of future control strategies.

Asunto(s)

Alelos; Bencimidazoles/farmacología; Resistencia a Medicamentos; Genotipo; Necator americanus/efectos de los fármacos; Necator americanus/genética; Polimorfismo de Nucleótido Simple; Tubulina (Proteína)/genética; Animales; Brasil/epidemiología; Humanos; Necatoriasis/epidemiología; Necatoriasis/parasitología; Vigilancia en Salud Pública

Palabras clave

ARMS-PCR; Beta-tubulin; Drug resistance; Necator americanus

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Bencimidazoles / Resistencia a Medicamentos / Necator americanus / Polimorfismo de Nucleótido Simple / Alelos / Genotipo Tipo de estudio: Risk_factors_studies Límite: Animals / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Infect Genet Evol Asunto de la revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos

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