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Deletion of growth hormone receptor in hypothalamic neurons affects the adaptation capacity to aerobic exercise.
Pedroso, João A B; Dos Santos, Lucas B P; Furigo, Isadora C; Spagnol, Alexandre R; Wasinski, Frederick; List, Edward O; Kopchick, John J; Donato, Jose.
Afiliación
  • Pedroso JAB; Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Departamento de Fisiologia e Biofisica, Sao Paulo, 05508-000, Brazil.
  • Dos Santos LBP; Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Departamento de Fisiologia e Biofisica, Sao Paulo, 05508-000, Brazil.
  • Furigo IC; Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Departamento de Fisiologia e Biofisica, Sao Paulo, 05508-000, Brazil.
  • Spagnol AR; Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Departamento de Farmacologia, Sao Paulo, 05508-000, Brazil.
  • Wasinski F; Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Departamento de Fisiologia e Biofisica, Sao Paulo, 05508-000, Brazil.
  • List EO; Edison Biotechnology Institute and Heritage College of Osteopathic Medicine, Ohio University, Athens, OH, 45701, USA.
  • Kopchick JJ; Edison Biotechnology Institute and Heritage College of Osteopathic Medicine, Ohio University, Athens, OH, 45701, USA.
  • Donato J; Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Departamento de Fisiologia e Biofisica, Sao Paulo, 05508-000, Brazil. Electronic address: jdonato@icb.usp.br.
Peptides ; 135: 170426, 2021 01.
Article en En | MEDLINE | ID: mdl-33069692
The hypothalamus mediates important exercise-induced metabolic adaptations, possibly via hormonal signals. Hypothalamic leptin receptor (LepR)- and steroidogenic factor 1 (SF1)-expressing neurons are directly responsive to growth hormone (GH) and deletion of GH receptor (GHR) in these cells impairs neuroendocrine responses during situations of metabolic stress. In the present study, we determined whether GHR ablation in LepR- or SF1-expressing cells modifies acute and chronic metabolic adaptations to exercise. Male mice carrying deletion of GHR in LepR- or SF1-expressing cells were submitted to 8 weeks of treadmill running training. Changes in aerobic performance and exercise-induced metabolic adaptations were determined. Mice carrying GHR deletion in LepR cells showed increased aerobic performance after 8 weeks of treadmill training, whereas GHR ablation in SF1 cells prevented improvement in running capacity. Trained mice carrying GHR ablation in SF1 cells exhibited increased fat mass and reduced cross-sectional area of the gastrocnemius muscle. In contrast, deletion of GHR in LepR cells reduced fat mass and increased gastrocnemius muscle hypertrophy, energy expenditure and voluntary locomotor activity in trained mice. Although glucose tolerance was not significantly affected by targeted deletions, glycemia before and immediately after maximum running tests was altered by GHR ablation. In conclusion, GHR signaling in hypothalamic neurons regulates the adaptation capacity to aerobic exercise in a cell-specific manner. These findings suggest that GH may represent a hormonal cue that informs specific hypothalamic neurons to produce exercise-induced acute and chronic metabolic adaptations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Receptores de Somatotropina / Ejercicio Físico / Receptores de Leptina / Factor Esteroidogénico 1 Límite: Animals / Humans / Male Idioma: En Revista: Peptides Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Receptores de Somatotropina / Ejercicio Físico / Receptores de Leptina / Factor Esteroidogénico 1 Límite: Animals / Humans / Male Idioma: En Revista: Peptides Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos