Your browser doesn't support javascript.
loading
Association between Mitochondrial DNA Sequence Variants and V˙O2 max Trainability.
Vellers, Heather L; Verhein, Kirsten C; Burkholder, Adam B; Lee, Jaehoon; Kim, Youngmin; Lightfoot, J Timothy; Shi, Min; Weinberg, Clarice R; Sarzynski, Mark A; Bouchard, Claude; Kleeberger, Steven R.
Afiliación
  • Vellers HL; Texas Tech University, Lubbock, TX.
  • Verhein KC; National Institute of Environmental Health Sciences, Research Triangle Park, NC.
  • Burkholder AB; National Institute of Environmental Health Sciences, Research Triangle Park, NC.
  • Lee J; Texas Tech University, Lubbock, TX.
  • Kim Y; Texas Tech University, Lubbock, TX.
  • Lightfoot JT; Texas A&M University, College Station, TX.
  • Shi M; National Institute of Environmental Health Sciences, Research Triangle Park, NC.
  • Weinberg CR; National Institute of Environmental Health Sciences, Research Triangle Park, NC.
  • Sarzynski MA; University of South Carolina, Columbia, SC.
  • Bouchard C; Pennington Biomedical Center, Baton Rouge, LA.
  • Kleeberger SR; National Institute of Environmental Health Sciences, Research Triangle Park, NC.
Med Sci Sports Exerc ; 52(11): 2303-2309, 2020 11.
Article en En | MEDLINE | ID: mdl-33064405
PURPOSE: We designed the study to determine whether mitochondrial DNA (mtDNA) haplogroup, sequence, and heteroplasmy differed between individuals previously characterized as low (LR) or high responders (HR) as defined by their maximal oxygen uptake response to a standardized aerobic exercise training program. METHODS: DNA was isolated from whole blood in subjects from the HERITAGE Family Study that were determined to be either HR (n = 15) or LR (n = 15). mtDNA was amplified by long-range polymerase chain reaction, then tagged with Nextera libraries and sequenced on a MiSeq instrument. RESULTS: Different mtDNA haplogroup subtypes were found in HR and LR individuals. Compared with HR subjects, significantly more LR subjects had variants in 13 sites, including 7 in hypervariable (HV) regions: HV2 (G185A: 0 vs 6, P = 0.02; G228A: 0 vs 5, P = 0.04; C295T: 0 vs 6; P = 0.04), HV3 (C462T: 0 vs 5, P = 0.04; T489C: 0 vs 5; P = 0.04), and HV1 (C16068T: 0 vs 6, P = 0.02; T16125C: 0 vs 6, P = 0.02). Remaining variants were in protein coding genes, mtND1 (1 vs 8, P = 0.02), mtND3 (A10397G: 0 vs 5, P = 0.04), mtND4 (A11250G: 1 vs 8, P = 0.02), mtND5 (G13707A: 0 vs 5, P = 0.04), and mtCYTB (T14797C: 0 vs 5, P = 0.04; C15451A: 1 vs 8, P = 0.02). Average total numbers of heteroplasmies (P = 0.83) and frequency of heteroplasmies (P = 0.05) were similar between the groups. CONCLUSIONS: Our findings provide specific sites across the mitochondrial genome that may be related to maximal oxygen uptake trainability.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Consumo de Oxígeno / ADN Mitocondrial / Ejercicio Físico / Genoma Mitocondrial Tipo de estudio: Risk_factors_studies Límite: Adolescent / Adult / Humans / Male Idioma: En Revista: Med Sci Sports Exerc Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Consumo de Oxígeno / ADN Mitocondrial / Ejercicio Físico / Genoma Mitocondrial Tipo de estudio: Risk_factors_studies Límite: Adolescent / Adult / Humans / Male Idioma: En Revista: Med Sci Sports Exerc Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos