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Clinical implications of SARS-CoV-2 cycle threshold values in solid organ transplant recipients.
Gaston, David C; Malinis, Maricar; Osborn, Rebecca; Peaper, David R; Landry, Marie; Juthani-Mehta, Manisha; Azar, Marwan M.
Afiliación
  • Gaston DC; Section of Infectious Diseases, Yale University School of Medicine, New Haven, Connecticut.
  • Malinis M; Section of Infectious Diseases, Yale University School of Medicine, New Haven, Connecticut.
  • Osborn R; Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut.
  • Peaper DR; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut.
  • Landry M; Section of Infectious Diseases, Yale University School of Medicine, New Haven, Connecticut.
  • Juthani-Mehta M; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut.
  • Azar MM; Section of Infectious Diseases, Yale University School of Medicine, New Haven, Connecticut.
Am J Transplant ; 21(3): 1304-1311, 2021 03.
Article en En | MEDLINE | ID: mdl-33043603
Detection of SARS-CoV-2 viral RNA by RT-PCR assays is the primary diagnostic test for COVID-19. Cycle threshold (CT ) values generated by some of these assays provide inversely proportional proxy measurements of viral load. The clinical implications of CT values are incompletely characterized, particularly in solid organ transplant (SOT) recipients. We conducted a retrospective chart review of 25 adult SOT recipients admitted to the Yale New Haven Health System between March 1 and May 15, 2020, analyzing 50 test results to investigate the clinical implications of SARS-CoV-2 CT values in this population. Initial CT values from upper respiratory tract samples were significantly higher in patients on tacrolimus, but were not associated with admission severity nor highest clinical acuity. Viral RNA was detected up to 38 days from symptom onset with a gradual increase in CT values over time. In five patients with serial testing, CT values <35.0 were detected >21 days after symptom onset in 4/5 and ≥27 days in 2/5, demonstrating prolonged RNA detection. These data describe SARS-CoV-2 viral dynamics in SOT patients and suggest that CT values may not be useful to predict COVID-19 severity in SOT patients. SARS-CoV-2 CT values may be more useful in informing infection prevention measures.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Órganos / Carga Viral / Pandemias / Receptores de Trasplantes / SARS-CoV-2 / COVID-19 Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Am J Transplant Asunto de la revista: TRANSPLANTE Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Órganos / Carga Viral / Pandemias / Receptores de Trasplantes / SARS-CoV-2 / COVID-19 Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Am J Transplant Asunto de la revista: TRANSPLANTE Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos