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Optimizing the Decellularized Porcine Liver Scaffold Protocol.
Alaby Pinheiro Faccioli, Lanuza; Suhett Dias, Grazielle; Hoff, Victor; Lemos Dias, Marlon; Ferreira Pimentel, Cibele; Hochman-Mendez, Camila; Braz Parente, Daniella; Labrunie, Ester; Souza Mourão, Paulo Antonio; Rogério de Oliveira Salvalaggio, Paolo; Goldberg, Anna Carla; Campos de Carvalho, Antonio Carlos; Dos Santos Goldenberg, Regina Coeli.
Afiliación
  • Alaby Pinheiro Faccioli L; Cellular and Molecular Cardiology Laboratory, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Suhett Dias G; Radiology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Hoff V; Cellular and Molecular Cardiology Laboratory, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Lemos Dias M; Research and Education Institute, Hospital Israelita Albert Einstein, São Paulo, Brazil.
  • Ferreira Pimentel C; Cellular and Molecular Cardiology Laboratory, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Hochman-Mendez C; Cellular and Molecular Cardiology Laboratory, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Braz Parente D; Cellular and Molecular Cardiology Laboratory, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Labrunie E; Texas Heart Institute, Houston, Texas, USA.
  • Souza Mourão PA; Radiology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Rogério de Oliveira Salvalaggio P; D'Or Institute for Research and Education, Botafogo, Rio de Janeiro, Brazil.
  • Goldberg AC; Radiology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Campos de Carvalho AC; Connective Tissue Laboratory, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Dos Santos Goldenberg RC; Research and Education Institute, Hospital Israelita Albert Einstein, São Paulo, Brazil.
Cells Tissues Organs ; 211(4): 385-394, 2022.
Article en En | MEDLINE | ID: mdl-33040059
There are few existing methods for shortening the decellularization period for a human-sized whole-liver scaffold. Here, we describe a protocol that enables effective decellularization of the liver obtained from pigs weigh 120 ± 4.2 kg within 72 h. Porcine livers (approx. 1.5 kg) were decellularized for 3 days using a combination of chemical and enzymatic decellularization agents. After trypsin, sodium deoxycholate, and Triton X-100 perfusion, the porcine livers were completely translucent. Our protocol was efficient to promote cell removal, the preservation of extracellular matrix (ECM) components, and vascular tree integrity. In conclusion, our protocol is efficient to promote human-sized whole-liver scaffold decellularization and thus useful to generate bioengineered livers to overcome the shortage of organs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido Límite: Animals / Humans Idioma: En Revista: Cells Tissues Organs Asunto de la revista: ANATOMIA Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido Límite: Animals / Humans Idioma: En Revista: Cells Tissues Organs Asunto de la revista: ANATOMIA Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza