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Molecular and cellular effects of insulin-like growth factor-1 and LongR3-IGF-1 on in vitro maturation of bovine oocytes: comparative study.
Araujo, M S; Guastali, M D; Paulini, F; Silva, A N; Tsunemi, M H; Fontes, P K; Castilho, A C S; Landim-Alvarenga, F C.
Afiliación
  • Araujo MS; Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil.
  • Guastali MD; Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil.
  • Paulini F; Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil.
  • Silva AN; School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
  • Tsunemi MH; Department of Biostatistics, Biosciences Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
  • Fontes PK; Department of Pharmacology, Biosciences Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
  • Castilho ACS; Department of Pharmacology, Biosciences Institute, São Paulo State University, Botucatu, São Paulo, Brazil.
  • Landim-Alvarenga FC; Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil. Electronic address: fernanda.landim@unesp.br.
Growth Horm IGF Res ; 55: 101357, 2020 12.
Article en En | MEDLINE | ID: mdl-33038561
Addition effects of insulin-like growth factor-1 (IGF-1) and its synthetic analogue insulin-like growth factor-1 recombinant-3 (LongR3-IGF-1) after in vitro maturation (IVM) of cattle cumulus-oocyte complexes (COCs) were compared and evaluated on meiotic progression, apoptosis and profile genes of oocyte competence (GDF9, BMP15, BAX, BCL2, OOSP1, IGFBP2, IGBFP4 and IGFBP5), and their respective cumulus cells (AREG, EGFR, FSHR, COX2, BAX, BCL2, IGFBP2, IGFBP4 and IGFBP5). The 739 COCs (n = 10 pools) of bovine ovaries were collected, selected and matured with IGF-1 (100 ng/mL), LongR3-IGF-1 (100 ng/mL), and in two control groups with 0.1% polyvinyl alcohol (PVA) or 10% fetal bovine serum (FBS), for 22-24 h. The statistical analysis was performed by a linear mixed effects model, ANOVA and Tukey tests. There was no statistical difference between experimental groups taken into account the meiotic progression and apoptosis (P > 0.05). Nevertheless, there were statistical differences (P ≤ 0.05) among FBS, IGF-1 and LongR3-IGF-1 groups for IGFBP4 gene expression, and among PVA, IGF-1 and LongR3-IGF-1 for COX2 gene expression in cumulus cells. Moreover, statistical difference was found for BCL2 gene expression between IGF-1, FBS and PVA groups and for IGFBP4 gene expression between LongR3-IGF-1, PVA and FBS in oocytes. There was no statistical difference between experimental groups for other genes evaluated. These results showed a good performance of IVM of bovine oocytes in the presence of LongR3-IGF-1 and the possibility of replacement of IGF-1 and FBS.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Proteínas Recombinantes / Factor I del Crecimiento Similar a la Insulina / Células del Cúmulo / Técnicas de Maduración In Vitro de los Oocitos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Growth Horm IGF Res Asunto de la revista: ENDOCRINOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Proteínas Recombinantes / Factor I del Crecimiento Similar a la Insulina / Células del Cúmulo / Técnicas de Maduración In Vitro de los Oocitos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Growth Horm IGF Res Asunto de la revista: ENDOCRINOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido