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Dehydrated Caenorhabditis elegans Stocks Are Resistant to Multiple Freeze-Thaw Cycles.
McClanahan, Patrick D; McCloskey, Richard J; Ng Tung Hing, Melanie; Raizen, David M; Fang-Yen, Christopher.
Afiliación
  • McClanahan PD; Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
  • McCloskey RJ; Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
  • Ng Tung Hing M; Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
  • Raizen DM; Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
  • Fang-Yen C; Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
G3 (Bethesda) ; 10(12): 4505-4512, 2020 12 03.
Article en En | MEDLINE | ID: mdl-33033066
Ultracold preservation is widely used for storage of genetic stocks of Caenorhabditis elegans Current cryopreservation protocols are vulnerable to refrigeration failures, which can result in the loss of stock viability due to damage during re-freezing. Here we present a method for preserving worms in a dehydrated and frozen form that retains viability after multiple freeze-thaw cycles. After dehydration in the presence of trehalose or glycerol, C. elegans stocks can be frozen and thawed multiple times while maintaining viability. While both dauer and non-dauer larvae survive desiccation and freezing, the dauer defective mutant daf-16 does not survive desiccation. Our technique is useful for storing stocks in a manner robust to freezer failures, and potentially for shipping strains between laboratories.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trehalosa / Caenorhabditis elegans Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trehalosa / Caenorhabditis elegans Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido