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Multimodal imaging reveals a unique autofluorescence signature of Randall's plaque.
Winfree, Seth; Weiler, Courtney; Bledsoe, Sharon B; Gardner, Tony; Sommer, André J; Evan, Andrew P; Lingeman, James E; Krambeck, Amy E; Worcester, Elaine M; El-Achkar, Tarek M; Williams, James C.
Afiliación
  • Winfree S; Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Weiler C; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Bledsoe SB; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Gardner T; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Sommer AJ; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Evan AP; Molecular Microspectroscopy Laboratory, Department of Chemistry and Biochemistry, Miami University, Oxford, OH, USA.
  • Lingeman JE; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Krambeck AE; Department of Urology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Worcester EM; Department of Urology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • El-Achkar TM; Division of Nephrology, Department of Medicine, University of Chicago, Chicago, IL, USA.
  • Williams JC; Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Urolithiasis ; 49(2): 123-135, 2021 Apr.
Article en En | MEDLINE | ID: mdl-33026465
Kidney stones frequently develop as an overgrowth on Randall's plaque (RP) which is formed in the papillary interstitium. The organic composition of RP is distinct from stone matrix in that RP contains fibrillar collagen; RP in tissue has also been shown to have two proteins that are also found in stones, but otherwise the molecular constituents of RP are unstudied. We hypothesized that RP contains unique organic molecules that can be differentiated from the stone overgrowth by fluorescence. To test this, we used micro-CT-guided polishing to expose the interior of kidney stones for multimodal imaging with multiphoton, confocal and infrared microscopy. We detected a blue autofluorescence signature unique to RP, the specificity of which was also confirmed in papillary tissue from patients with stone disease. High-resolution mineral mapping of the stone also showed a transition from the apatite within RP to the calcium oxalate in the overgrowth, demonstrating the molecular and spatial transition from the tissue to the urine. This work provides a systematic and practical approach to uncover specific fluorescence signatures which correlate with mineral type, verifies previous observations regarding mineral overgrowth onto RP and identifies a novel autofluorescence signature of RP demonstrating RP's unique molecular composition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apatitas / Oxalato de Calcio / Cálculos Renales / Imagen Óptica / Médula Renal Límite: Female / Humans / Male Idioma: En Revista: Urolithiasis Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apatitas / Oxalato de Calcio / Cálculos Renales / Imagen Óptica / Médula Renal Límite: Female / Humans / Male Idioma: En Revista: Urolithiasis Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania