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Ciliate Diversity From Aquatic Environments in the Brazilian Atlantic Forest as Revealed by High-Throughput DNA Sequencing.
Fernandes, Noemi M; Campello-Nunes, Pedro H; Paiva, Thiago S; Soares, Carlos A G; Silva-Neto, Inácio D.
Afiliación
  • Fernandes NM; Departamento de Zoologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil. noemi.mfernandes@gmail.com.
  • Campello-Nunes PH; Departamento de Zoologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
  • Paiva TS; Departamento de Zoologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
  • Soares CAG; Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
  • Silva-Neto ID; Departamento de Zoologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
Microb Ecol ; 81(3): 630-643, 2021 Apr.
Article en En | MEDLINE | ID: mdl-33025060
Rainforest aquatic ecosystems include complex habitats with scarce information on their unicellular eukaryote diversity and community structure. We have investigated the diversity of ciliates in freshwater and brackish environments along the Brazilian Atlantic Forest, based on the hypervariable V4 region of the 18S-rDNA obtained by high-throughput DNA sequencing. Our analyses detected 409 ciliate taxonomic units (OTUs), mostly attributed to the classes Oligohymenophorea and Spirotrichea. A total of 11 classes, 12 subclasses, 112 genera, and 144 species were reported. We found the following: (a) the ciliate communities are more diverse in freshwater- than in Atlantic Forest-associated brackish environments; (b) the ciliate communities are composed by a small amount of highly abundant OTUs, but a high number of low-abundant or rare OTUs; (c) nearly one-third of the ciliate OTUs share less than 97% sequence identity to reference sequences and (d) phylogenetic inference supports the hypothesis that the V4 region of the Ciliophora 18S-rDNA is a suitable marker for accurate evolutionary inferences at class level. Our results showed that a considerable fraction of the HTS-detected diversity of ciliates from Brazilian Atlantic Forest is not represented in the currently available molecular databases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cilióforos / Ecosistema País/Región como asunto: America do sul / Brasil Idioma: En Revista: Microb Ecol Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cilióforos / Ecosistema País/Región como asunto: America do sul / Brasil Idioma: En Revista: Microb Ecol Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos